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Invitrogen
SP100 Polyclonal Antibody
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Application
FIGURE: 1 / 2
SP100 Antibody (PA5-78177) in ICC/IF
SP100 Polyclonal Antibody detects SP100 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 Polyclonal Antibody (Product # PA5-78177) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Product Details
PA5-78177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP100. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.76 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736651
Product Specific Information
Positive Control: Jurkat, Raji, NCI-H929
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686E07254; FLJ00340; FLJ34579; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP 100; SP100-HMG nuclear autoantigen; Speckled 100 kDa
Gene Aliases: lysp100b; SP100
UniProt ID: (Human) P23497
Entrez Gene ID: (Human) 6672
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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