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Application
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SPG7 Antibody (PA5-87745) in WB
Western blot analysis of SPG7 in extracts of various cell lines. Samples were incubated with SPG7 Polyclonal antibody (Product # PA5-87745) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-87745
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPG7 (NP_0031101)
偶联物
Unconjugated
形式
Liquid
浓度
0.32 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2804382
产品详细信息
Immunogen sequence: MAVLLLLLRA LRRGPGPGPR PLWGPGPAWS PGFPARPGRG RPYMASRPPG DLAEAGGRAL QSLQLRLLTP TFEGINGLLL KQHLVQNPVR LWQLLGGTFY FNTSRLKQKN KEKDKSKGKA PEEDEEERRR RERDDQMYRE RLRTLLVIAV VMSLLNALST SGGSISWNDF VHEMLAKGEV QRVQVVPESD VVEVYLHPGA VVFGRPRLAL MYRMQVANID KFEEKLRAAE DELNIEAKDR IPVSYKRTGF; Positive Samples: U-87MG, MCF7, HT-29, K-562, Mouse brain, Mouse kidney, Mouse liver; Cellular Location: Mitochondrion membrane, Multi-pass membrane protein
靶标信息
This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cell adhesion regulator; Cell matrix adhesion regulator; Mitochondrial inner membrane m-AAA protease component paraplegin; Paraplegin; paraplegin, isoform 1; spastic paraplegia 7 (pure and complicated autosomal recessive); Spastic paraplegia 7 protein
基因别名: AI452278; AU015315; CAR; CMAR; PGN; SPG5C; SPG7
UniProt ID: (Human) Q9UQ90, (Mouse) Q3ULF4
Entrez Gene ID: (Human) 6687, (Mouse) 234847
