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Invitrogen
SPO11 Polyclonal Antibody
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Application
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SPO11 Antibody (PA5-99106) in WB
Western Blot analysis of SPO11 using a SPO11 Polyclonal antibody (Product # PA5-99106) at a concentration of 5.8 µg/mL. Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 45 kDa.
Product Details
PA5-99106
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Meiotic recombination protein SPO11 protein (28-109AA)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.87 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2813719
Target Information
Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cancer/testis antigen 35; CT35; Meiotic recombination protein SPO11; MGC39953; spermatogenesis associated 43; SPO11 meiotic protein covalently bound to DSB homolog
Gene Aliases: CT35; SPATA43; SPO11; TOPVIA
UniProt ID: (Human) Q9Y5K1
Entrez Gene ID: (Human) 23626
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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