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Application
FIGURE: 1 / 1
SPSB1 Antibody (PA5-89504) in WB
Western blot analysis of SPSB1 in extracts of various cell lines. Samples were incubated with SPSB1 Polyclonal antibody (Product # PA5-89504) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89504
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human SPSB1 (NP_0793822)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.83 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV QLLHSWNNND; Positive Samples: U-87MG, A-549, A-375, Mouse spleen, Rat testis; Cellular Location: Cytoplasm
Target Information
Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CBG; novel SPRY domain containing protein; SPRY domain-containing SOCS box protein 1; SPRY domain-containing SOCS box protein SSB-1; SSB-1
Gene Aliases: 1110014L01Rik; 4930422J18Rik; AI596360; AI854583; RGD1309319; SPSB1; SSB-1; SSB1
UniProt ID: (Human) Q96BD6, (Mouse) Q9D5L7
Entrez Gene ID: (Human) 80176, (Rat) 313722, (Mouse) 74646
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