Invitrogen

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eFluor™ 660, eBioscience™

SSEA1 Antibody in Immunocytochemistry (ICC/IF)

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SSEA1 Antibody (50-8813-42) in ICC/IF

Nature 2017 - Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Immunocytochemistry

SSEA1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

SSEA1 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

SSEA1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

50-8813-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgM

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMC-480 (MC-480)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11219681

Product Specific Information

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of the F9 cell line. This can be used at 5 µL (0.06 µg µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells.

Cell reports methods

Iwatsuki K,Oikawa M,Kobayashi H,Penfold CA,Sanbo M,Yamamoto T,Hochi S,Kurimoto K,Hirabayashi M,Kobayashi T

50-8813-42 was used in Flow Cytometry to produce authentic primordial germ cell-like cells that undergo functional gametogenesis leading to the birth of viable offspring.

Mon Aug 28 00:00:00 EDT 2023

Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells.

The EMBO journal

Severino J,Bauer M,Mattimoe T,Arecco N,Cozzuto L,Lorden P,Hamada N,Nosaka Y,Nagaoka SI,Audergon P,Tarruell A,Heyn H,Hayashi K,Saitou M,Payer B

50-8813-42 was used in Flow cytometry/Cell sorting to investigate X-inactivation and reactivation dynamics using a tailor-made in vitro system of primordial germ cell-like cell (PGCLC) differentiation from mouse embryonic stem cells.

Tue Jun 14 00:00:00 EDT 2022

Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability.

Nature cell biology

Monserrat J,Morales Torres C,Richardson L,Wilson TS,Patel H,Domart MC,Horswell S,Song OR,Jiang M,Crawford M,Bui M,Dalal Y,Scaffidi P

50-8813-42 was used in Flow Cytometry to demonstrate that targeting MSL (male-specific lethal complex) may be a valuable means to increase CIN (chromosomal instability) beyond the level tolerated by cancer cells without inducing severe adverse effects in normal tissues.

Thu Apr 01 00:00:00 EDT 2021

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

50-8813-42 was used in Flow Cytometry to open the feasibility of utilizing haEpiSCs to elucidate fundamental biological processes including cell fate alternations.

Sat May 01 00:00:00 EDT 2021

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

50-8813-42 was used in Flow Cytometry to open the feasibility of utilizing haEpiSCs to elucidate fundamental biological processes including cell fate alternations.

Sat May 01 00:00:00 EDT 2021

Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming.

STAR protocols

Ying Z,Liu Z,Xiang G,Xin Y,Wang J,Liu X

50-8813-42 was used in Flow Cytometry to describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope.

Fri Jun 18 00:00:00 EDT 2021

Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation.

Nature communications

Bauer M,Vidal E,Zorita E,Üresin N,Pinter SF,Filion GJ,Payer B

50-8813-42 was used in Flow Cytometry to investigate if topologically associating domains formation and transcriptional reactivation are causally independent during X-reactivation while establishing Xist as a common denominator.

Wed Jun 09 00:00:00 EDT 2021

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Wed May 06 00:00:00 EDT 2020

Functional Oocytes Derived from Granulosa Cells.

Cell reports

Tian C,Liu L,Ye X,Fu H,Sheng X,Wang L,Wang H,Heng D,Liu L

50-8813-42 was used in Flow cytometry/Cell sorting to demonstrate that funtional oocytes posessing high genomic stability can be produced in vitro from somatic gPSCs through cellular reprogramming, facilitated by protonic sodium or acid - overall a safe and effective chemical induction technique.

Tue Dec 24 00:00:00 EST 2019

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Nature

Stadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Immunocytochemistry

Thu Sep 28 00:00:00 EDT 2017

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Nature

Yamaji M,Jishage M,Meyer C,Suryawanshi H,Der E,Yamaji M,Garzia A,Morozov P,Manickavel S,McFarland HL,Roeder RG,Hafner M,Tuschl T

50-8813 was used in Flow cytometry/Cell sorting to investigate the molecular function of DND1 protein.

Thu Mar 23 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Not All H3K4 Methylations Are Created Equal: Mll2/COMPASS Dependency in Primordial Germ Cell Specification.

Molecular cell

Hu D,Gao X,Cao K,Morgan MA,Mas G,Smith ER,Volk AG,Bartom ET,Crispino JD,Di Croce L,Shilatifard A

50-8813 was used in Flow cytometry/Cell sorting to analyse the contribution of MLL2's methyltransferase and CXXC domain in H3K4 trimethylation in mouse embryonic stem cells.

Thu Feb 02 00:00:00 EST 2017

Not All H3K4 Methylations Are Created Equal: Mll2/COMPASS Dependency in Primordial Germ Cell Specification.

Molecular cell

Hu D,Gao X,Cao K,Morgan MA,Mas G,Smith ER,Volk AG,Bartom ET,Crispino JD,Di Croce L,Shilatifard A

50-8813 was used in Flow cytometry/Cell sorting to analyse the contribution of MLL2's methyltransferase and CXXC domain in H3K4 trimethylation in mouse embryonic stem cells.

Thu Feb 02 00:00:00 EST 2017

A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation.

The Journal of experimental medicine

McGarvey AC,Rybtsov S,Souilhol C,Tamagno S,Rice R,Hills D,Godwin D,Rice D,Tomlinson SR,Medvinsky A

50-8813-42 was used in Immunohistochemistry to identify novel secreted regulators of haematopoietic stem cell maturation.

Mon Dec 04 00:00:00 EST 2017

Noncanonical function of DGCR8 controls mESC exit from pluripotency.

The Journal of cell biology

Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C

50-8813 was used in Flow cytometry/Cell sorting to reveal a new noncanonical function of DGCR8 in the modulation of the alternative splicing of Tcf7l1 mRNA in addition to its established function in microRNA biogenesis.

Wed Feb 01 00:00:00 EST 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2016

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2016

Foxd3 Promotes Exit from Naive Pluripotency through Enhancer Decommissioning and Inhibits Germline Specification.

Cell stem cell

Respuela P,Nikolić M,Tan M,Frommolt P,Zhao Y,Wysocka J,Rada-Iglesias A

50-8813 was used in Flow cytometry/Cell sorting to discover a cycle of activation and deactivation of Foxd3 required for exit from naive pluripotency and primordial germ cell specification.

Thu Jan 07 00:00:00 EST 2016

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Immunohistochemistry

Thu Jul 07 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

An alternative pluripotent state confers interspecies chimaeric competency.

Nature

Wu J,Okamura D,Li M,Suzuki K,Luo C,Ma L,He Y,Li Z,Benner C,Tamura I,Krause MN,Nery JR,Du T,Zhang Z,Hishida T,Takahashi Y,Aizawa E,Kim NY,Lajara J,Guillen P,Campistol JM,Esteban CR,Ross PJ,Saghatelian A,Ren B,Ecker JR,Izpisua Belmonte JC

50-8813 was used in Flow cytometry/Cell sorting to show modulation of culture parameters efficiently selects for region-selective pluripotent stem cells from mouse embryos and primate pluripotent stem cells.

Thu May 21 00:00:00 EDT 2015

Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.

Cell stem cell

Pardo-Saganta A,Law BM,Tata PR,Villoria J,Saez B,Mou H,Zhao R,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Immunofluorescence

Thu Feb 05 00:00:00 EST 2015

Coexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme.

Oncotarget

Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Fri Mar 20 00:00:00 EDT 2015

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Molecular cell

Das PP,Shao Z,Beyaz S,Apostolou E,Pinello L,De Los Angeles A,O'Brien K,Atsma JM,Fujiwara Y,Nguyen M,Ljuboja D,Guo G,Woo A,Yuan GC,Onder T,Daley G,Hochedlinger K,Kim J,Orkin SH

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jan 09 00:00:00 EST 2014

Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.

Nature communications

Zhang J,Liu G,Ruan Y,Wang J,Zhao K,Wan Y,Liu B,Zheng H,Peng T,Wu W,He P,Hu FQ,Jian R

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Mon Oct 06 00:00:00 EDT 2014

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Nature cell biology

ten Berge D,Kurek D,Blauwkamp T,Koole W,Maas A,Eroglu E,Siu RK,Nusse R

Published figure using SSEA1 monoclonal antibody (Product # 50-8813-42) in Flow Cytometry

Sun Aug 14 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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Function(s)

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eFluor™ 660, eBioscience™

SSEA1 Antibody (50-8813-42) in ICC/IF

Product Details

50-8813-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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