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Invitrogen
SSH3 Polyclonal Antibody
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Application
FIGURE: 1 / 7
SSH3 Antibody (PA5-101788) in ICC/IF
Immunofluorescent analysis of SSH3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SSH3 polyclonal antibody (Product # PA5-101788) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101788
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SSH3(Accession Q8TE77), corresponding to amino acid residues T356-G406.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851220
Product Specific Information
Antibody detects endogenous levels of total SSH3.
Target Information
The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSSH-3L; Protein phosphatase Slingshot homolog 3; slingshot 3; slingshot homolog 3; slingshot-3L; slingshot-like 3; SSH-3L; SSH-like protein 3
Gene Aliases: BC028922; mSSH-3L; SSH-3; SSH3; SSH3L
UniProt ID: (Human) Q8TE77, (Mouse) Q8K330, (Rat) Q5XIS1
Entrez Gene ID: (Human) 54961, (Mouse) 245857, (Rat) 365396
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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