Proteintech

STAT3 Monoclonal Antibody (3G2D12)

STAT3 Antibody in Immunocytochemistry (ICC/IF)

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STAT3 Antibody (60199-1-IG) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 60199-1-IG (STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

STAT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STAT3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

60199-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:20,000

View 51 publications 51 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat, Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3G2D12

Immunogen

STAT3 Fusion Protein Ag0360 (2-219 aa encoded by BC000627)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Immunogen sequence: AQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVS (2-219 aa encoded by BC000627 )

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aging-accelerated differential production and aggregation of STAT3 protein in neuronal cells and neural stem cells in the male mouse spinal cord.

Biogerontology

Zhao T,Liu C,Liu L,Wang X,Liu C

Wed Feb 01 00:00:00 EST 2023

Aging-accelerated differential production and aggregation of STAT3 protein in neuronal cells and neural stem cells in the male mouse spinal cord.

Biogerontology

Zhao T,Liu C,Liu L,Wang X,Liu C

Wed Feb 01 00:00:00 EST 2023

Aging-accelerated differential production and aggregation of STAT3 protein in neuronal cells and neural stem cells in the male mouse spinal cord.

Biogerontology

Zhao T,Liu C,Liu L,Wang X,Liu C

Wed Feb 01 00:00:00 EST 2023

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xian Z,Tian J,Zhao Y,Yi Y,Li C,Han J,Zhang Y,Wang Y,Wang L,Liu S,Pan C,Liu C,Wang D,Meng J,Tang X,Wang F,Liang A

Thu Jun 01 00:00:00 EDT 2023

4-Octyl itaconate attenuates LPS-induced acute kidney injury by activating Nrf2 and inhibiting STAT3 signaling.

Molecular medicine (Cambridge, Mass.)

Xu L,Cai J,Li C,Yang M,Duan T,Zhao Q,Xi Y,Sun L,He L,Tang C,Sun L

Mon Apr 24 00:00:00 EDT 2023

A novel GRK2 inhibitor alleviates experimental arthritis through restraining Th17 cell differentiation.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Tao J,Jiang C,Guo P,Chen H,Zhu Z,Su T,Zhou W,Tai Y,Han C,Ma Y,Chen J,Sun W,Wang Y,Wei W,Wang Q

Sun Jan 01 00:00:00 EST 2023

LC-MS Analysis of Ginsenosides in Different Parts of Panax quinquefolius and Their Potential for Coronary Disease Improvement.

Planta medica

Zhang X,Kong C,Wang X,Hou H,Yu H,Wang L,Li P,Li X,Zhang Y,Han L,Liu K

Thu Jun 01 00:00:00 EDT 2023

Pan-cancer analysis of PCAT6 and its effect on oesophageal squamous cell carcinoma cell proliferation and migration.

Medical oncology (Northwood, London, England)

Luo Q,Kuang Y,Tao X,Zhu X,Zhao X,Lu G,Zhang W,Sun E,Chen B

Wed Mar 22 00:00:00 EDT 2023

Nifuroxazide Activates the Parthanatos to Overcome TMPRSS2:ERG Fusion-Positive Prostate Cancer.

Molecular cancer therapeutics

Li C,Zhang J,Wu Q,Kumar A,Pan G,Kelvin DJ

Thu Mar 02 00:00:00 EST 2023

Sclareol attenuates liver fibrosis through SENP1-mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling.

Phytotherapy research : PTR

Ge MX,Niu WX,Bao YY,Lu ZN,He HW

Fri Sep 01 00:00:00 EDT 2023

A Bioinspired Manganese-Organic Framework Ameliorates Ischemic Stroke through its Intrinsic Nanozyme Activity and Upregulating Endogenous Antioxidant Enzymes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang J,Wang Y,Xiaohalati X,Su Q,Liu J,Cai B,Yang W,Wang Z,Wang L

Sat Jul 01 00:00:00 EDT 2023

STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma.

Cancer immunology, immunotherapy : CII

Cui H,Lan Z,Zou KL,Zhao YY,Yu GT

Mon May 01 00:00:00 EDT 2023

STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma.

Cancer immunology, immunotherapy : CII

Cui H,Lan Z,Zou KL,Zhao YY,Yu GT

Mon May 01 00:00:00 EDT 2023

5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I.

Frontiers of medicine

Wu C,Liu D,Zhang L,Wang J,Ding Y,Sun Z,Wang W

Thu Jun 01 00:00:00 EDT 2023

Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma.

Journal of ethnopharmacology

Jie XL,Luo ZR,Yu J,Tong ZR,Li QQ,Wu JH,Tao Y,Feng PS,Lan JP,Wang P

Tue Dec 05 00:00:00 EST 2023

POR overexpression induces tamoxifen-resistance in breast cancer through the STAT1/c-Myc pathway.

Molecular carcinogenesis

Chen S,Wu D,Liu Q,Jin F,Yao F,Fang Y

Wed Feb 01 00:00:00 EST 2023

Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression.

Clinical and experimental pharmacology & physiology

Yang F,Li L,Zhou Y,Pan W,Liang X,Huang L,Huang J,Cheng Y,Geng L,Xu W,Gong S

Thu Jun 01 00:00:00 EDT 2023

Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression.

Clinical and experimental pharmacology & physiology

Yang F,Li L,Zhou Y,Pan W,Liang X,Huang L,Huang J,Cheng Y,Geng L,Xu W,Gong S

Thu Jun 01 00:00:00 EDT 2023

Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression.

Clinical and experimental pharmacology & physiology

Yang F,Li L,Zhou Y,Pan W,Liang X,Huang L,Huang J,Cheng Y,Geng L,Xu W,Gong S

Thu Jun 01 00:00:00 EDT 2023

Fetal anemia and elevated hepcidin in a mouse model of fetal alcohol spectrum disorder.

Pediatric research

Helfrich KK,Saini N,Kwan STC,Rivera OC,Mooney SM,Smith SM

Tue Aug 01 00:00:00 EDT 2023

Exosomal lncRNA HOTAIR induces PDL1⁺ B cells to impede anti-tumor immunity in colorectal cancer.

Biochemical and biophysical research communications

Xie Z,Xia J,Jiao M,Zhao P,Wang Z,Lin S,Xing Y,Li Y,Lu Z,Zhong Z,Miao C,Zhou P,Qian J,Wang L,Zhang D,Gu J,Chu Y,Liu R

Sun Feb 12 00:00:00 EST 2023

Exosomal lncRNA HOTAIR induces PDL1⁺ B cells to impede anti-tumor immunity in colorectal cancer.

Biochemical and biophysical research communications

Xie Z,Xia J,Jiao M,Zhao P,Wang Z,Lin S,Xing Y,Li Y,Lu Z,Zhong Z,Miao C,Zhou P,Qian J,Wang L,Zhang D,Gu J,Chu Y,Liu R

Sun Feb 12 00:00:00 EST 2023

Anemoside B4 protects against chronic relapsing colitis in mice by modulating inflammatory response, colonic transcriptome and the gut microbiota.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Han Q,Deng LR,Zou M,Tang HZ,Huang CY,Chen FJ,Tomlinson B,Li YH

Tue Nov 01 00:00:00 EDT 2022

YTHDF1 Negatively Regulates Treponema pallidum-Induced Inflammation in THP-1 Macrophages by Promoting SOCS3 Translation in an m6A-Dependent Manner.

Frontiers in immunology

Li Z,Teng M,Jiang Y,Zhang L,Luo X,Liao Y,Yang B

Fri Apr 22 00:00:00 EDT 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Pharmaceuticals (Basel, Switzerland)

Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 EST 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Pharmaceuticals (Basel, Switzerland)

Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 EST 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Pharmaceuticals (Basel, Switzerland)

Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 EST 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Pharmaceuticals (Basel, Switzerland)

Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 EST 2022

Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways.

Cell biology and toxicology

Wang H,Ai J,Shopit A,Niu M,Ahmed N,Tesfaldet T,Tang Z,Li X,Jamalat Y,Chu P,Peng J,Ma X,Qaed E,Han G,Zhang W,Wang J,Tang Z

Wed Jun 01 00:00:00 EDT 2022

KGF-2 Regulates STAP-2-Mediated Signal Transducer and Activator of Transcription 3 Signaling and Reduces Skin Scar Formation.

The Journal of investigative dermatology

Zhou Q,Gong J,Bi J,Yang X,Zhang L,Lu C,Li L,Chen M,Cai J,Yang R,Li X,Li Z,Wang X

Fri Jul 01 00:00:00 EDT 2022

KGF-2 Regulates STAP-2-Mediated Signal Transducer and Activator of Transcription 3 Signaling and Reduces Skin Scar Formation.

The Journal of investigative dermatology

Zhou Q,Gong J,Bi J,Yang X,Zhang L,Lu C,Li L,Chen M,Cai J,Yang R,Li X,Li Z,Wang X

Fri Jul 01 00:00:00 EDT 2022

Mucin 16 Promotes Colorectal Cancer Development and Progression Through Activation of Janus Kinase 2.

Digestive diseases and sciences

Liu Z,Gu Y,Li X,Zhou L,Cheng X,Jiang H,Huang Y,Zhang Y,Xu T,Yang W,Huang Q

Wed Jun 01 00:00:00 EDT 2022

Dihydromyricetin Alleviates Pulmonary Fibrosis by Regulating Abnormal Fibroblasts Through the STAT3/p-STAT3/GLUT1 Signaling Pathway.

Frontiers in pharmacology

Li Z,Geng J,Xie B,He J,Wang J,Peng L,Hu Y,Dai H,Wang C

Sat Apr 02 00:00:00 EDT 2022

Nicotinamide N-Methyltransferase Remodeled Cell Metabolism and Aggravated Proinflammatory Responses by Activating STAT3/IL1β/PGE₂ Pathway.

ACS omega

Yang C,Wang T,Zhu S,Zong Z,Luo C,Zhao Y,Liu J,Li T,Liu X,Liu C,Deng H

Tue Oct 25 00:00:00 EDT 2022

Exposure to short-chain chlorinated paraffins induces astrocyte activation via JAK2/STAT3 signaling pathway.

Ecotoxicology and environmental safety