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Product Details
60199-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3G2D12
Immunogen
STAT3 Fusion Protein Ag0360 (2-219 aa encoded by BC000627)
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
Immunogen sequence: AQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVS (2-219 aa encoded by BC000627 )
Target Information
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT
Gene Aliases: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3
UniProt ID: (Human) P40763, (Rat) P52631, (Mouse) P42227
Entrez Gene ID: (Human) 6774, (Rat) 25125, (Mouse) 20848
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II repressing transcription factor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
signal transducer activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
protein kinase binding
protein phosphatase binding
chromatin DNA binding
CCR5 chemokine receptor binding
glucocorticoid receptor binding
identical protein binding
transcription regulatory region DNA binding
protein dimerization activity
RNA polymerase II transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
temperature homeostasis
eye photoreceptor cell differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein import into nucleus
movement of cell or subcellular component
acute-phase response
signal transduction
JAK-STAT cascade
nervous system development
aging
cell proliferation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of hydrogen peroxide biosynthetic process
viral process
phosphorylation
cytokine-mediated signaling pathway
sexual reproduction
intracellular receptor signaling pathway
response to estradiol
cellular response to hormone stimulus
leptin-mediated signaling pathway
somatic stem cell population maintenance
miRNA mediated inhibition of translation
regulation of multicellular organism growth
response to drug
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
eating behavior
negative regulation of apoptotic process
cellular response to leptin stimulus
response to leptin
response to ethanol
positive regulation of Notch signaling pathway
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of mitochondrial membrane permeability
astrocyte differentiation
regulation of cell cycle
radial glial cell differentiation
regulation of feeding behavior
growth hormone receptor signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
interleukin-6-mediated signaling pathway
cellular response to organic cyclic compound
energy homeostasis
negative regulation of neuron death
positive regulation of growth factor dependent skeletal muscle satellite cell proliferation
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of metalloendopeptidase activity
positive regulation of gene silencing by miRNA
positive regulation of ATP biosynthetic process
negative regulation of neuron migration
response to organic substance
response to organic cyclic compound
stem cell population maintenance
response to cytokine
response to peptide hormone
negative regulation of cell death
transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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