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Product Details
MA5-42852
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H10O9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 649-748 of human STAT4 (NP_0031421)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911993
Product Specific Information
Positive test controls include: HeLa, PC-3, Jurkat. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: MAENIPENPL KYLYPDIPKD KAFGKHYSSQ PCEVSRPTER GDKGYVPSVF IPISTIRSDS TEPHSPSDLL PMSPSVYAVL RENLSPTTIE TAMKSPYSAE
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIS4; HSPC060; OTTHUMP00000163553; Signal transducer and activator of transcription 4; SOCS4; SOCS6; SSI4; STAI4; STAT; STATI4
Gene Aliases: SLEB11; STAT4
UniProt ID: (Human) Q14765, (Mouse) P42228
Entrez Gene ID: (Human) 6775, (Rat) 367264, (Mouse) 20849
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