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Invitrogen
SV40 Large T-Antigen Polyclonal Antibody
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Application
FIGURE: 1 / 1
SV40 Large T-Antigen Antibody (PA5-112036) in WB
Western Blot analysis of SV40 Large T-Antigen in various whole cell extract. Sample was separated with 7.5% SDS-PAGE, incubated with polyclonal antibody (Product # PA5-112036) at a dilution of 1:1,000, and followed by HRP-conjugated anti-rabbit IgG.
Product Details
PA5-112036
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of SV40 Large T antigen (SV40 virus (strain 776)). The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866772
Target Information
The SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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