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Application
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Sulfotyrosine Antibody (MA5-27551) in ICC/IF
Immunofluorescent analysis of Sulfotyrosine in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Sulfotyrosine monoclonal antibody (Product # MA5-27551) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min at ... View More
Product Details
MA5-27551
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7C5
Immunogen
Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735376
Product Specific Information
A 1:1,000 dilution of MA5-27551 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Information
Sulfotyrosine is involved in protein-protein interactions. Sulfotyrosines are present in all multicellular eukaryotes, but unicellular eukaryotes such as yeast and prokaryotes are not capable of tyrosine sulfation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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