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Invitrogen
Synaptophysin Polyclonal Antibody
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Application
FIGURE: 1 / 19
Synaptophysin Antibody (PA5-27286) in ICC/IF
Synaptophysin Polyclonal Antibody detects Synaptophysin protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptophysin stained by Synaptophysin Polyclonal Antibody (Product # PA5-27286) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-27286
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.65 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544762
Product Specific Information
Recommended positive controls: human brain, mouse brain, rat brain, Mouse small intestine, LNCaP.
Predicted reactivity: Mouse (94%), Rat (95%), Rhesus Monkey (97%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BM89 antigen; Major synaptic vesicle protein p38; Synaptophysin; Syp I
Gene Aliases: A230093K24Rik; AI848995; MRX96; MRXSYP; p38; Syn; SYP; Syp1
UniProt ID: (Human) P08247, (Rat) P07825, (Mouse) Q62277
Entrez Gene ID: (Human) 6855, (Rat) 24804, (Mouse) 20977
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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