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Invitrogen

Syndecan 2 Polyclonal Antibody

2 Published Figures

6 References

View all (11) Syndecan 2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

Application

Syndecan 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Syndecan 2 Antibody (36-6200) in ICC/IF

Immunofluorescence analysis of Syndecan-2 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Syndecan-2 Rabbit polyclonal Antibody (Product # 36-6200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temp... View More

Syndecan 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Syndecan 2 Antibody in Western Blot (WB)

Syndecan 2 Antibody in Immunohistochemistry (IHC)

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Product Details

36-6200

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mammal, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533270

Target Information

Syndecans are heparan sulfate proteoglycans (HSPGs) that are involved in cell adhesion, tissue morphogenesis, differentiation, and act as low affinity co-receptors of soluble growth factors. It is involved in the mitogenic activity and signaling of Granulocyte-Macrophage Colony-stimulating factor in osteoblasts, and has been shown to have a regulatory role in matrix assembly. Syndecan-2 is expressed at epithelial-mesenchymal interfaces, and prechondrogenic and preosteogenic mesenchymal condensations, which are areas of high morphogenetic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression.

The Journal of biological chemistry

Beauvais DM,Nelson SE,Adams KM,Stueven NA,Jung O,Rapraeger AC

36-6200 was used in Flow Cytometry to add to the growing list of receptor tyrosine kinases that support tumorigenesis when activated by their association with syndecans at sites of matrix adhesion and identify new potential targets for cancer therapy.

Wed Jun 01 00:00:00 UTC 2022

Osteoblastic heparan sulfate glycosaminoglycans control bone remodeling by regulating Wnt signaling and the crosstalk between bone surface and marrow cells.

Cell death & disease

Mansouri R,Jouan Y,Hay E,Blin-Wakkach C,Frain M,Ostertag A,Le Henaff C,Marty C,Geoffroy V,Marie PJ,Cohen-Solal M,Modrowski D

Published figure using Syndecan 2 polyclonal antibody (Product # 36-6200) in Immunohistochemistry

Thu Jun 29 00:00:00 UTC 2017

Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody.

Scientific reports

Park H,Kim M,Kim HJ,Lee Y,Seo Y,Pham CD,Lee J,Byun SJ,Kwon MH

36-6200 was used in Western Blotting to show that anti-DNA antibodies enter cells via both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans simultaneously.

Mon Oct 30 00:00:00 UTC 2017

Syndecan-2 is a key regulator of transforming growth factor beta 2/Smad2-mediated adhesion in fibrosarcoma cells.

IUBMB life

Mytilinaiou M,Bano A,Nikitovic D,Berdiaki A,Voudouri K,Kalogeraki A,Karamanos NK,Tzanakakis GN

36-6200 was used in western blot to test if syndecan-2 affects TGFβ2 functions.

Fri Feb 01 00:00:00 UTC 2013

Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery.

Acta obstetricia et gynecologica Scandinavica

Akerud A,Dubicke A,Sennstrom M,Ekman-Ordeberg G,Malmstrom A

36-6200 was used in immunocytochemistry to determine if pregnancy alters proteoglycan production in cervical fibroblasts

Mon Dec 29 00:00:00 UTC 2008

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CD362; cell surface-associated heparan sulfate proteoglycan 1; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; SYND2; syndecan proteoglycan 2; Syndecan-2

Gene Aliases: CD362; HSPG; HSPG1; SDC2; SYND2

UniProt ID: (Human) P34741

Entrez Gene ID: (Human) 6383

Function(s)

Process(es)

retinoid metabolic process response to hypoxia glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process biological_process bioluminescence cell migration glycosaminoglycan metabolic process response to caffeine wound healing ephrin receptor signaling pathway dendrite morphogenesis regulation of dendrite morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Syndecan 2 Polyclonal Antibody

Syndecan 2 Antibody (36-6200) in ICC/IF

Product Details

36-6200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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