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Application
FIGURE: 1 / 1
TAF13 Antibody (PA5-97233) in WB
Western blot analysis of TAF13 in extracts of various cell lines. Samples were incubated with TAF13 Polyclonal antibody (Product # PA5-97233) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-97233
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human TAF13 (NP_0056361)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809035
Product Specific Information
Immunogen sequence: MADEEEDPTF EEENEEIGGG AEGGQGKRKR LFSKELRCMM YGFGDDQNPY TESVDILEDL VIEFITEMTH KAMSIGRQGR VQVEDIVFLI RKDPRKFARV KDLLTMNEEL KRARKAFDEA NYGS; Positive Samples: BxPC-3, Mouse pancreas, Rat liver; Cellular Location: Nucleus
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit associated with a subset of TFIID complexes. This subunit interacts with TBP and with two other small subunits of TFIID, TAF10 and TAF11. There is a pseudogene located on chromosome 6. [provided by RefSeq, Jul 2008].
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC22425; TAF(II)18; TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 18kDa; TAF13 RNA polymerase II, TATA box-binding protein-associated factor; TAFII-18; TATA box binding protein (TBP)-associated factor, RNA polymerase II, K, 18kD; TATA box binding protein associated factor 13; TBP-associated factor, RNA polymerase II, K, 18kD; transcription initiation factor TFIID 18 kD subunit; Transcription initiation factor TFIID 18 kDa subunit; Transcription initiation factor TFIID subunit 13
Gene Aliases: 1810004N01Rik; 2010309N11Rik; AI847295; TAF(II)18; TAF13; TAF2K; TAFII-18; TAFII18
UniProt ID: (Human) Q15543, (Mouse) P61216
Entrez Gene ID: (Human) 6884, (Mouse) 99730, (Rat) 310784
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