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            • Primary Antibodies ›
            • TAF4 Antibodies

            Invitrogen

            TAF4 Monoclonal Antibody (20TA-1B12)

            View all (6) TAF4 antibodies
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            Cite TAF4 Monoclonal Antibody (20TA-1B12)

            • Antibody Testing Data (2)
            Application
            TAF4 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            TAF4 Antibody (MA3-075) in ICC/IF

            Immunofluorescent analysis of TAF4 in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a TAF4 mouse monoclonal antibody (Product # MA3-075) at a dilution of 1:4000 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilut... View More
            View Product

            TAF4 Antibody in Immunocytochemistry (ICC/IF)
            TAF4 Antibody in Western Blot (WB)

            Product Details

            MA3-075

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            -

            Immunocytochemistry (ICC/IF)

            1:4,000
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            20TA-1B12

            Immunogen

            Recombinant TAF4 protein. Epitope is localized between aa 815 and 836.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            Conc. Not Determined

            Storage buffer

            ascites

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2637134

            Product Specific Information

            MA3-075 detects TATA-Binding Protein (TBP) Associated factor 4 from human and mouse samples.

            MA3-075 has been successfully used in Western blot and immunofluorescence applications.

            The MA3-075 immunogen is a recombinant TAF4 protein. Epitope is localized between aa 815 and 836.

            Target Information

            TAF II p105, also called TAF4B, is a celltype specific transcriptional co-activator that is a component of the TFIID complex. Expressed primarily in B cells and ovarian granulosa cells, TAF II p105 can interact with OCBA/POU2AF1 to activate B cell-specific octamerdependent transcription. Additionally, TAF II p105 plays an important role in co-activating the transcription factor NFkB and is essential for activation of anti-apoptotic genes such as TNFAIP3. TAF II p135, also known as TAF4, TAF2C, TAF2C1, TAF4A or TAFII130, is a 1,085 amino acid subunit of TFIID that accelerates transcriptional activation triggered by thyroid hormone (TR) or retinoic acid (RA). Localized to the nucleus, TAF II p135 contains one TAFH domain and is thought to bind to proteins that contain glutamine-rich domains, such as the transcription factor CREB.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: FLJ41943; RNA polymerase II TBP-associated factor subunit C; TAF(II)130; TAF(II)135; TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa; TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD; TAFII 130; TAFII 135; TAFII-130; TAFII-135; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; TATA box binding protein associated factor 4; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; Transcription initiation factor TFIID 135 kDa subunit; Transcription initiation factor TFIID subunit 4

            View more View less

            Gene Aliases: 135kDa; AI450312; TAF2C; TAF2C1; TAF4; TAF4A; TAFII130; TAFII135

            View more View less

            UniProt ID: (Human) O00268

            View more View less

            Entrez Gene ID: (Human) 6874, (Mouse) 228980

            View more View less

            Function(s)

            DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding protein heterodimerization activity general transcription factor

            Process(es)

            ovarian follicle development DNA-templated transcription, initiation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter viral process positive regulation of transcription, DNA-templated regulation of signal transduction by p53 class mediator
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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