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Application
FIGURE: 1 / 1
TAF6 Antibody (MA3-077) in WB
Western blot analysis of TAF6 was performed by loading 20 µg of HeLa and T47D nuclear lysates, 20 µg of MCF7 and F9 whole cell lysates, and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF6 was detected at ~80 kDa using a TAF6 mouse monoclonal antibody (Product # MA3-077) at a dilutio... View More
Product Details
MA3-077
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
25TA-2G7
Immunogen
Synthetic peptide coupled to ovalalbumin, from aa 39 to aa 56
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633322
Product Specific Information
MA3-077 detects TATA Binding protein (TBP) Associated factor 6 in human and mouse samples.
MA3-077 has been successfully used in a Western blot application.
The MA3-077 immunogen is a synthetic peptide coupled to ovalalbumin, from aa 39 to aa 56.
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781E21155; p80; RNA polymerase II TBP-associated factor subunit E; TAF(II)70; TAF(II)80; TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kDa; TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa; TAFII-70; TAFII-80; TAFII80; TATA box binding protein (Tbp)-associated factor, RNA polymerase II, E; TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD; TATA box binding protein associated factor 6; Transcription initiation factor TFIID 70 kDa subunit; Transcription initiation factor TFIID 80 kDa subunit; Transcription initiation factor TFIID subunit 6
Gene Aliases: 80kDa; AW549759; MGC:8964; p80; TAF(II)70; TAF(II)80; TAF2E; TAF6; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
UniProt ID: (Human) P49848, (Mouse) Q62311
Entrez Gene ID: (Human) 6878, (Mouse) 21343
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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