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Invitrogen
TCHP Polyclonal Antibody
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Application
FIGURE: 1 / 2
TCHP Antibody (PA5-100790) in ICC/IF
Immunocytochemistry analysis of TCHP in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TCHP polyclonal antibody (Product # PA5-100790) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100790
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TCHP(Accession Q9BT92), corresponding to amino acid residues E460-N498.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850293
Product Specific Information
Antibody detects endogenous levels of total TCHP.
Target Information
TCHP may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. This protein may regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitochondrial protein with oncostatic activity; Mitostatin; Protein TCHP; TCHP; Trichoplein keratin filament-binding protein; trichoplein, keratin filament binding; Tumor suppressor protein
Gene Aliases: TCHP; TpMs
UniProt ID: (Human) Q9BT92
Entrez Gene ID: (Human) 84260
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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