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TECK Antibody (500-P134BT-25UG) in WB
Western Blot: To detect hTECK by Western Blot analysis TECK Polyclonal Antibody, Biotin (Product # 500-P134BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTECK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P134BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 14.2 kDa Recombinant Human TECK (CCL25)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929417
Product Specific Information
AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TECK (CCL25). Anti-Human TECK (CCL25)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TECK (CCL25) (500-P134) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL25).
Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTECK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P134BT-1MG will be provided as 2 x 500 µg
Target Information
Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine. TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors. TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes. Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECKMGC150327; TECKvar; thymus expressed chemokine; Thymus-expressed chemokine
Gene Aliases: CCL25; Ckb15; SCYA25; TECK
UniProt ID: (Human) O15444
Entrez Gene ID: (Human) 6370
positive regulation of cell-matrix adhesion
monocyte chemotaxis
chemotaxis
inflammatory response
immune response
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
negative regulation of leukocyte tethering or rolling
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