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Invitrogen
TECK Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
TECK Antibody (PA5-47479) in Neu
Neutralization of TECK in human CCR9. Samples were incubated in TECK polyclonal antibody (Product # PA5-47479). Recombinant Human CCL25/TECK chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL25/TECK (3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL25/TECK Antigen Affinity-purified Polyclonal An... View More
Product Details
PA5-47479
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CCL25/TECK Gln24-Leu150
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610344
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CCL25/TECK is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine. TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors. TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes. Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECKMGC150327; TECKvar; thymus expressed chemokine; Thymus-expressed chemokine
Gene Aliases: CCL25; Ckb15; SCYA25; TECK
UniProt ID: (Human) O15444
Entrez Gene ID: (Human) 6370
positive regulation of cell-matrix adhesion
monocyte chemotaxis
chemotaxis
inflammatory response
immune response
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
negative regulation of leukocyte tethering or rolling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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