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            • Primary Antibodies ›
            • RRN3 Antibodies

            Bethyl Laboratories

            TIF-IA Polyclonal Antibody

            View all (10) RRN3 antibodies
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            Catalog # A303-065A
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            Price (USD)
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            Cite TIF-IA Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            TIF-IA Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            TIF-IA Antibody (A303-065A) in WB

            Detection of human TIF-IA by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TIF-IA antibody (Product # A303-065A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. TIF-IA was also immunoprecipitated by rabbit anti-TIF-IA antibody BL11066, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B... View More
            View Product

            TIF-IA Antibody in Western Blot (WB)

            Product Details

            A303-065A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-5 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 601 and 651 of Human Transcription Initiation Factor IA.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            tris citrate/phosphate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Based on 100% sequence identity, this antibody is predicted to react with Orangutan

            Target Information

            Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog; TIF-IA; Transcription initiation factor IA; transcription initiation factor TIF-IA

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            Gene Aliases: A-270G1.2; RRN3; TIFIA

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            UniProt ID: (Human) Q9NYV6

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            Entrez Gene ID: (Human) 54700

            View more View less

            Function(s)

            RNA polymerase I CORE element sequence-specific DNA binding RNA polymerase binding general transcription factor

            Process(es)

            in utero embryonic development transcription initiation from RNA polymerase I promoter nucleolus organization cytoplasm organization cell proliferation positive regulation of neuron projection development ribosome biogenesis positive regulation of transcription, DNA-templated homeostasis of number of cells negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of DNA-templated transcription, initiation
            It has to be done as per old AB suggested Products section.
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