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TIMP-2 Antibody (BS-10395R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-2 Polyclonal Antibody (bs-10395R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product Details
BS-10395R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human TIMP-2, amino acids 51-150.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSC-21K; Metalloproteinase inhibitor 2; testicular secretory protein Li 57; TIMP 2; TIMP-2; tissue inhibitor of metalloproteinase 2; Tissue inhibitor of metalloproteinases 2
Gene Aliases: CSC-21K; D11Bwg1104e; DDC8; Timp-2; TIMP2
UniProt ID: (Human) P16035, (Rat) P30121
Entrez Gene ID: (Human) 7077, (Rat) 29543, (Mouse) 21858
central nervous system development
aging
negative regulation of cell proliferation
response to hormone
negative regulation of endopeptidase activity
extracellular matrix disassembly
regulation of Rap protein signal transduction
response to cytokine
response to drug
positive regulation of MAPK cascade
positive regulation of neuron differentiation
positive regulation of adenylate cyclase activity
negative regulation of mitotic cell cycle
negative regulation of Ras protein signal transduction
negative regulation of membrane protein ectodomain proteolysis
spermatogenesis
regulation of cAMP metabolic process
positive regulation of catalytic activity
negative regulation of catalytic activity
regulation of MAPK cascade
regulation of neuron differentiation
negative regulation of proteolysis
cellular response to organic substance
negative regulation of peptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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