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Product Details
BS-12392R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human TLE5/Amino-terminal enhancer of split, amino acids 21-120.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amino enhancer of split; Amino-terminal enhancer of split; Gp130-associated protein GAM; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG; R-esp1; related to Drosophila groucho; TLE family member 5; TLE family member 5, transcriptional modulator
Gene Aliases: AES; AES-1; AES-2; AL024115; ESP1; GRG; Grg-5; GRG5; TLE5
UniProt ID: (Human) Q08117, (Mouse) P63002, (Rat) P63003
Entrez Gene ID: (Human) 166, (Mouse) 14797, (Rat) 29466
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
multicellular organism development
organ morphogenesis
negative regulation of gene expression
Wnt signaling pathway
negative regulation of protein binding
negative regulation of transcription, DNA-templated
negative regulation of response to cytokine stimulus
response to interleukin-1
negative regulation of canonical Wnt signaling pathway
positive regulation of anoikis
skeletal system development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to extracellular stimulus
regulation of growth
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