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Invitrogen
TNRC6A Polyclonal Antibody
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Application
FIGURE: 1 / 5
TNRC6A Antibody (PA5-115336) in ICC/IF
Immunocytochemistry analysis of TNRC6A in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115336) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115336
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TNRC6A(Accession Q8NDV7), corresponding to amino acid residues R1596-V1646.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899972
Product Specific Information
Antibody detects endogenous levels of total GW182.
Target Information
TNRC6A is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAG repeat protein 26; CTD-2540M10.1; DKFZp666E117; EDIE; EMSY interactor protein; FLJ22043; Glycine-tryptophan protein of 182 kDa; GW182 autoantigen; MGC75384; Protein GW1; trinucleotide repeat containing 6; Trinucleotide repeat-containing gene 6A protein
Gene Aliases: 2010321I05Rik; 3110054G10Rik; AW557223; CAGH26; D130023A07Rik; GW1; GW182; KIAA1460; TNRC6; TNRC6A
UniProt ID: (Human) Q658L5, (Mouse) Q3UHK8
Entrez Gene ID: (Human) 27327, (Rat) 308971, (Mouse) 233833
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