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Invitrogen

TOX Monoclonal Antibody (TXRX10), PE, eBioscience™

8 Published Figures

11 References

View all (11) TOX antibodies

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Protocols

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Catalog # 12-6502-82

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

Application

FIGURE: 1 / 9

TOX Antibody (12-6502-82) in Flow

C57Bl/6 thymocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82), followed by intracellular staining with 0.5 µg of Anti-Human/Mouse TOX PE using the Foxp3 Staining Buffer Set and protocol (Product # 00-5521-00). The histogram (right) demonstrates TOX staining of CD4+CD8+ (blue histogram), CD4loCD8lo (green histogram), and CD4+CD8lo (purple) thymocytes. Cell populations were gated as indicated on the dot plot (left). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-6502-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TXRX10

Conjugate

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10855034

Product Specific Information

Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4+ T cell development as a regulator of the CD4loCD8lo to CD4+CD8lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection.

Applications Reported: This TXRX10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TXRX10 antibody has been tested intracellular flow cytometric analysis of mouse thymocytes using the Foxp3 Buffers (Product # 00-5521-00) and protocol. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Tumor-secreted IFI35 promotes proliferation and cytotoxic activity of CD8⁺ T cells through PI3K/AKT/mTOR signaling pathway in colorectal cancer.

Journal of biomedical science

Li P,Zhou D,Chen D,Cheng Y,Chen Y,Lin Z,Zhang X,Huang Z,Cai J,Huang W,Lin Y,Ke H,Long J,Zou Y,Ye S,Lan P

Published figure using TOX monoclonal antibody (Product # 12-6502-82) in Flow Cytometry

Wed Jun 28 00:00:00 UTC 2023

Trans-vaccenic acid reprograms CD8⁺ T cells and anti-tumour immunity.

Nature

Fan H,Xia S,Xiang J,Li Y,Ross MO,Lim SA,Yang F,Tu J,Xie L,Dougherty U,Zhang FQ,Zheng Z,Zhang R,Wu R,Dong L,Su R,Chen X,Althaus T,Riedell PA,Jonker PB,Muir A,Lesinski GB,Rafiq S,Dhodapkar MV,Stock W,Odenike O,Patel AA,Opferman J,Tsuji T,Matsuzaki J,Shah H,Faubert B,Elf SE,Layden B,Bissonnette BM,He YY,Kline J,Mao H,Odunsi K,Gao X,Chi H,He C,Chen J

Published figure using TOX monoclonal antibody (Product # 12-6502-82) in Flow Cytometry

Wed Nov 01 00:00:00 UTC 2023

Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness.

Nature metabolism

Cheng H,Qiu Y,Xu Y,Chen L,Ma K,Tao M,Frankiw L,Yin H,Xie E,Pan X,Du J,Wang Z,Zhu W,Chen L,Zhang L,Li G

12-6502-82 was used in Flow cytometry/Cell sorting to reveal an unexpected capacity of extracellular acidosis to maintain the stem-like properties of T cells, but also advance our understanding of how methionine metabolism affects T cell stemness.

Wed Feb 01 00:00:00 UTC 2023

PD-1/PD-L1 blockade abrogates a dysfunctional innate-adaptive immune axis in critical β-coronavirus disease.

Science advances

Duhalde Vega M,Olivera D,Gastão Davanzo G,Bertullo M,Noya V,Fabiano de Souza G,Primon Muraro S,Castro I,Arévalo AP,Crispo M,Galliussi G,Russo S,Charbonnier D,Rammauro F,Jeldres M,Alamón C,Varela V,Batthyany C,Bollati-Fogolín M,Oppezzo P,Pritsch O,Proença-Módena JL,Nakaya HI,Trias E,Barbeito L,Anegon I,Cuturi MC,Moraes-Vieira P,Segovia M,Hill M

Published figure using TOX monoclonal antibody (Product # 12-6502-82) in Flow Cytometry

Fri Sep 23 00:00:00 UTC 2022

Functional assessment of the cell-autonomous role of NADase CD38 in regulating CD8⁺ T cell exhaustion.

iScience

Ma K,Sun L,Shen M,Zhang X,Xiao Z,Wang J,Liu X,Jiang K,Xiao-Feng Qin F,Guo F,Zhang B,Zhang L

Published figure using TOX monoclonal antibody (Product # 12-6502-82) in Flow Cytometry

Fri May 20 00:00:00 UTC 2022

Distinct phenotypic states and spatial distribution of CD8⁺ T cell clonotypes in human brain metastases.

Cell reports. Medicine

Sudmeier LJ,Hoang KB,Nduom EK,Wieland A,Neill SG,Schniederjan MJ,Ramalingam SS,Olson JJ,Ahmed R,Hudson WH

12-6502-82 was used in Flow Cytometry to identify signaling pathways within CD8+ T cells and in their surrounding environment that may be targeted for immunotherapy of brain metastases.

Tue May 17 00:00:00 UTC 2022

Characterization of the spectrum of trivalent VAV1-mutation-driven tumours using a gene-edited mouse model.

Molecular oncology

Robles-Valero J,Fernández-Nevado L,Cuadrado M,Lorenzo-Martín LF,Fernández-Pisonero I,Abad A,Redín E,Montuenga L,Martín-Zanca D,Bigas A,Mallo M,Dosil M,Bustelo XR

12-6502-82 was used in Flow cytometry/Cell sorting to indicate that VAV1 mutations play critical, although highly cell-type-specific, roles in tumourigenesis.

Sat Oct 01 00:00:00 UTC 2022

Uptake of oxidized lipids by the scavenger receptor CD36 promotes lipid peroxidation and dysfunction in CD8⁺ T cells in tumors.

Immunity

Xu S,Chaudhary O,Rodríguez-Morales P,Sun X,Chen D,Zappasodi R,Xu Z,Pinto AFM,Williams A,Schulze I,Farsakoglu Y,Varanasi SK,Low JS,Tang W,Wang H,McDonald B,Tripple V,Downes M,Evans RM,Abumrad NA,Merghoub T,Wolchok JD,Shokhirev MN,Ho PC,Witztum JL,Emu B,Cui G,Kaech SM

12-6502-82 was used in Flow cytometry/Cell sorting to conclude that an oxidized lipid-CD36 axis promotes intratumoral CD8+ T cell dysfunction and serves as a therapeutic avenue for immunotherapies.

Tue Jul 13 00:00:00 UTC 2021

Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesis.

The EMBO journal

Robles-Valero J,Fernández-Nevado L,Lorenzo-Martín LF,Cuadrado M,Fernández-Pisonero I,Rodríguez-Fdez S,Astorga-Simón EN,Abad A,Caloto R,Bustelo XR

Published figure using TOX monoclonal antibody (Product # 12-6502-82) in Flow Cytometry

Mon Nov 15 00:00:00 UTC 2021

Single-cell transcriptome analysis reveals TOX as a promoting factor for T cell exhaustion and a predictor for anti-PD-1 responses in human cancer.

Genome medicine

Kim K,Park S,Park SY,Kim G,Park SM,Cho JW,Kim DH,Park YM,Koh YW,Kim HR,Ha SJ,Lee I

12-6502 was used in Flow cytometry/Cell sorting to identify the regulatory factors involved in tumor-infiltrating CD8+ T cell exhaustion by analyzing the scRNA-seq data.

Fri Feb 28 00:00:00 UTC 2020

Landscape of Exhausted Virus-Specific CD8 T Cells in Chronic LCMV Infection.

Cell reports

Sandu I,Cerletti D,Oetiker N,Borsa M,Wagen F,Spadafora I,Welten SPM,Stolz U,Oxenius A,Claassen M

12-6502-82 was used in Flow Cytometry to reveal that exhausted cells are heterogeneous, adopt organ-specific transcriptomic profiles, and can be divided into five main functional subpopulations: advanced exhaustion, effector-like, intermediate, proliferating, or memory-like.

Tue Aug 25 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; Thymus high mobility group box protein TOX

Gene Aliases: 1700007F02Rik; KIAA0808; TOX; TOX1

UniProt ID: (Human) O94900, (Mouse) Q66JW3

Entrez Gene ID: (Human) 9760, (Mouse) 252838

Function(s)

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TOX Monoclonal Antibody (TXRX10), PE, eBioscience™

TOX Antibody (12-6502-82) in Flow

Product Details

12-6502-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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