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Invitrogen
TOX Monoclonal Antibody (TXRX10), eBioscience™
Application
FIGURE: 1 / 8
TOX Antibody (14-6502-82) in WB
Western Blot was performed using Anti-TOX Monoclonal Antibody (TXRX10), eBioscience™ (Product # 14-6502-80, 14-6502-82) and 57, 60 kDa bands corresponding to Thymocyte selection-associated high mobility group box protein TOX were observed. Nuclear enriched extracts (40 µg lysate) of Ramos (Lane 1), Daudi (Lane 2), Jurkat (Lane 3), SH-SY5Y (Lane 4), Raji (Lane 5) and tissue extracts (40 µg lysate) of Mouse Heart (Lane 6), Mouse Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved prot... View More
Product Details
14-6502-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
TXRX10
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10718252
Product Specific Information
Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4^+ T cell development as a regulator of the CD4^loCD8^lo to CD4^+CD8^lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection.
Applications Reported: This TXRX10 antibody has been reported for use in immunoprecipitation and immunoblotting (WB).
Applications Tested: This TXRX10 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse thymocytes at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; Thymus high mobility group box protein TOX
Gene Aliases: 1700007F02Rik; KIAA0808; TOX; TOX1
UniProt ID: (Human) O94900, (Mouse) Q66JW3
Entrez Gene ID: (Human) 9760, (Mouse) 252838
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