Loading ...

Invitrogen

TRIB3 Polyclonal Antibody

2 References

View all (35) TRIB3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

FIGURE: 1 / 2

TRIB3 Antibody (PA5-15480) in WB

Western Blot was performed using Anti-TRIB3 Polyclonal Antibody (Product # PA5-15480) and a 40 kDa band corresponding to Tribbles homolog 3 was observed across cell lines and tissue tested. The protein expression was found to be upregulated in Hep G2 upon treatment with Tunicamycin and TGF beta 1 treatments. Nuclear enriched extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Tunicamycin (2 µg/mL for 12 hrs) (Lane 2), Hep G2 treated with TGF beta 1 (10 ng/mL for 12 hrs) (Lane 3), MDA-MB-231 (Lane 4), MCF7 (Lane 5), SW480 (Lane ... View More

Product Image Gallery

Product Details

PA5-15480

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 301-331 amino acids from the C-terminal region of human TRIB3

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2209090

Target Information

A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologues of Tribbles (Trbs) have been identified that control mitogen-activated protein kinase (MAPK) activity. TRIB3 also known as TRB-3, SINK, and SKIP3 is a member of Trbs family. Overexpression of this protein inhibits NF-kB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but does not inhibit NF-kB translocation to the nucleus and binding to DNA. TRIB3/SKIP3 is also overexpressed in multiple human tumors and is regulated by hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.

Nature communications

Li K,Wang F,Yang ZN,Zhang TT,Yuan YF,Zhao CX,Yeerjiang Z,Cui B,Hua F,Lv XX,Zhang XW,Yu JJ,Liu SS,Yu JM,Shang S,Xiao Y,Hu ZW

PA5-15480 was used in Western Blotting to highlight a key mechanism for controlling MYC expression and a potential therapeutic option for treating lymphomas with high TRIB3-MYC expression.

Wed Dec 09 00:00:00 UTC 2020

TRIB3 supports breast cancer stemness by suppressing FOXO1 degradation and enhancing SOX2 transcription.

Nature communications

Yu JM,Sun W,Wang ZH,Liang X,Hua F,Li K,Lv XX,Zhang XW,Liu YY,Yu JJ,Liu SS,Shang S,Wang F,Yang ZN,Zhao CX,Hou XY,Li PP,Huang B,Cui B,Hu ZW

PA5-15480 was used in Western Blot to report that expression of the pseudokinase Tribble 3 (TRIB3) positively associates with breast cancer stemness and progression.

Mon Dec 16 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: DJ1103G7.3; induced in fatty liver dystrophy 2; neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; p65-interacting inhibitor of NF-kappa-B; p65-interacting inhibitor of NF-kappaB; SINK; TRB-3; Tribbles 3; Tribbles homolog 3

Gene Aliases: C20orf97; Ifld2; NIPK; SINK; SKIP3; TRB-3; TRB3; TRIB3

UniProt ID: (Human) Q96RU7, (Mouse) Q8K4K2

Entrez Gene ID: (Human) 57761, (Mouse) 228775

Function(s)

transcription corepressor activity protein kinase inhibitor activity protein binding ATP binding kinase activity protein kinase binding mitogen-activated protein kinase kinase binding ubiquitin protein ligase binding ubiquitin-protein transferase regulator activity protein kinase activity transferase activity, transferring phosphorus-containing groups enzyme binding non-receptor serine/threonine protein kinase

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity regulation of glucose transport positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus response to endoplasmic reticulum stress regulation of MAP kinase activity negative regulation of fat cell differentiation negative regulation of fatty acid biosynthetic process negative regulation of transcription, DNA-templated positive regulation of ubiquitin-protein transferase activity intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of transcription, DNA-templated apoptotic process positive regulation of protein catabolic process negative regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more