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Application
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TRIM28 Antibody (PA1-852) in ICC/IF
Immunofluorescent analysis of KAP1 (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a KAP1 polyclonal antibody (Product # PA1-852) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated... View More
Product Details
PA1-852
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant human KAP-1.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2219366
Product Specific Information
PA1-852 detects KAP-1 (Kruppel-associated box-associated protein 1) in human, mouse and rat samples.
PA1-852 has been successfully used in Western blot, ICC/IF and IHC (P) procedures. By Western blot, this antibody detects an ~87 kDa protein representing recombinant human KAP-1. This antibody also detects a smaller protein that is believed to be KAP-1 degradation product. In immunofluorescent applications, this antibody primarily reacts with mouse or rat and shows non-specific staining in HeLa cells.
The PA1-852 antigen is a recombinant human KAP-1.
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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