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Invitrogen

TSPO Polyclonal Antibody

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7 Published Figures

8 References

View all (22) TSPO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

TSPO Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

TSPO Antibody (PA5-75544) in ICC/IF

Immunofluorescence analysis of TSPO was performed using THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (Product # PA5-75544) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000... View More

TSPO Antibody in Immunohistochemistry (IHC)

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TSPO Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of TSPO was observed in THP1, Raw 264.7 in comparison to SK-O-V3, HeLa and HepG2 using TSPO Polyclonal Antibody (Product # PA5-75544) in Western Blot. {RE}

Product Details

PA5-75544

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 124-169 of Human TSPO.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2719272

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of translocator protein alleviates mechanical allodynia and bladder dysfunction in cyclophosphamide-induced cystitis through repression of BDNF-mediated neuroinflammation.

European journal of pain (London, England)

Huang Y,Su M,Zhang C,Zhan H,Yang F,Gao Z,Zhou X,Liu B

PA5-75544 was used in Immunohistochemistry to explore whether activation of TSPO attenuates mechanical allodynia and bladder dysfunction by regulating BDNF induced neuroinflammation in a CYP-induced cystitis model.

Fri Jul 01 00:00:00 UTC 2022

Activation of translocator protein alleviates mechanical allodynia and bladder dysfunction in cyclophosphamide-induced cystitis through repression of BDNF-mediated neuroinflammation.

European journal of pain (London, England)

Huang Y,Su M,Zhang C,Zhan H,Yang F,Gao Z,Zhou X,Liu B

Fri Jul 01 00:00:00 UTC 2022

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Sun Oct 17 00:00:00 UTC 2021

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Sun Oct 17 00:00:00 UTC 2021

Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes.

ACS omega

Tournier BB,Ceyzériat K,Bouteldja FN,Millet P

PA5-75544 was used in Western Blotting to determine the effects of Aβ, Tau, and TSPO antagonists on proliferation/cell death and TSPO polymerization in the C6 astrocytic cell line.

Tue Jul 27 00:00:00 UTC 2021

TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

Acta pharmacologica Sinica

Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Wed Jan 01 00:00:00 UTC 2020

TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

Acta pharmacologica Sinica

Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Wed Jan 01 00:00:00 UTC 2020

Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tong J,Williams B,Rusjan PM,Mizrahi R,Lacapère JJ,McCluskey T,Furukawa Y,Guttman M,Ang LC,Boileau I,Meyer JH,Kish SJ

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Fri May 01 00:00:00 UTC 2020

Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tong J,Williams B,Rusjan PM,Mizrahi R,Lacapère JJ,McCluskey T,Furukawa Y,Guttman M,Ang LC,Boileau I,Meyer JH,Kish SJ

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Western Blot

Fri May 01 00:00:00 UTC 2020

The peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone, ameliorates neurofunctional and neuroinflammatory abnormalities in a rat model of Gulf War Illness.

PloS one

Keledjian K,Tsymbalyuk O,Semick S,Moyer M,Negoita S,Kim K,Ivanova S,Gerzanich V,Simard JM

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Immunohistochemistry

Mon Jan 04 00:00:00 UTC 2021

The peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone, ameliorates neurofunctional and neuroinflammatory abnormalities in a rat model of Gulf War Illness.

PloS one

Keledjian K,Tsymbalyuk O,Semick S,Moyer M,Negoita S,Kim K,Ivanova S,Gerzanich V,Simard JM

Published figure using TSPO polyclonal antibody (Product # PA5-75544) in Immunohistochemistry

Mon Jan 04 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Benzodiazepin receptor (peripheral); benzodiazepine peripheral binding site; benzodiazepine receptor, peripheral; isoquinoline-binding protein; Mitochondrial benzodiazepine receptor; OTTHUMP00000028891; PBR; Peripheral-type benzodiazepine receptor; PKBS; Translocator protein; translocator protein (18kDa)

Gene Aliases: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; TSPO; Tspo1

UniProt ID: (Human) P30536, (Rat) P16257, (Mouse) P50637

Entrez Gene ID: (Human) 706, (Rat) 24230, (Mouse) 12257

Function(s)

androgen binding protein binding benzodiazepine receptor activity cholesterol binding ion channel binding

Process(es)

steroid biosynthetic process ion transport chloride transport lipid transport chemical synaptic transmission aging glial cell migration response to manganese ion response to vitamin B1 positive regulation of necrotic cell death peripheral nervous system axon regeneration adrenal gland development response to progesterone negative regulation of tumor necrosis factor production response to testosterone response to drug positive regulation of apoptotic process negative regulation of nitric oxide biosynthetic process response to pain behavioral response to pain response to axon injury regulation of steroid biosynthetic process positive regulation of mitochondrial depolarization positive regulation of calcium ion transport contact inhibition positive regulation of glial cell proliferation negative regulation of glial cell proliferation cellular response to lipopolysaccharide cellular response to zinc ion cellular hypotonic response positive regulation of reactive oxygen species metabolic process transport signal transduction protein targeting to mitochondrion heme biosynthetic process anion transport apoptotic process steroid metabolic process cell proliferation negative regulation of protein ubiquitination regulation of cholesterol transport maintenance of protein location in mitochondrion negative regulation of mitophagy negative regulation of ATP metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TSPO Polyclonal Antibody

TSPO Antibody (PA5-75544) in ICC/IF

Product Details

PA5-75544

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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TSPO Polyclonal Antibody

TSPO Antibody (PA5-75544) in ICC/IF

Product Details

PA5-75544

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Function(s)

Process(es)

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