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Invitrogen
TUBA1A Polyclonal Antibody
Application
FIGURE: 1 / 14
TUBA1A Antibody (PA5-22060) in ICC/IF
Immunofluorescence analysis of alpha Tubulin antibody was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-22060) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor®... View More
Product Details
PA5-22060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11154084
Product Specific Information
Recommended positive controls: Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, rat brain, Drosophila.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (98%), Pig (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1a antibody; alpha 3 antibody; Alpha-tubulin 1; Alpha-tubulin 3; alpha-tubulin 3 antibody; Alpha-tubulin isotype M-alpha-1; B-ALPHA-1 antibody; brain-specific antibody; FLJ25113 antibody; FLJ30169; H2 ALPHA; H2-ALPHA; H2ALPHA; hum-a-tub1; hum-a-tub1 antibody; hum-a-tub2; hum-a-tub2 antibody; LIS3 antibody; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SW-cl.56; OK/SWcl.56; Talpha1 alpha-tubulin; TUBA1A antibody; TUBA3 antibody; TUBB1; TUBB5; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1A chain antibody; Tubulin alpha-3 chain; tubulin alpha-3 chain antibody; Tubulin B-alpha-1; tubulin B-alpha-1 antibody; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific
Gene Aliases: B-ALPHA-1; LIS3; Tuba-1; Tuba1; TUBA1A; TUBA3
UniProt ID: (Human) Q71U36, (Mouse) P68369, (Rat) P68370
Entrez Gene ID: (Human) 7846, (Mouse) 22142, (Rat) 64158
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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