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Invitrogen
TWIST1 Monoclonal Antibody (2F8E7)
Application
FIGURE: 1 / 5
TWIST1 Antibody (MA5-17195) in ICC/IF
Immunofluorescence analysis of TWIST1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Anti-TWIST1 Mouse Monoclonal Antibody (2F8E7) (Product # MA5-17195) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
MA5-17195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2F8E7
Immunogen
Purified recombinant fragment of human TWIST1 (amino acids: 9-74) expressed in E. Coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538666
Product Specific Information
MA5-17195 targets TWIST1 in indirect ELISA, WB applications and shows reactivity with Human samples.
The MA5-17195 immunogen is purified recombinant fragment of human TWIST1 (amino acids: 9-74) expressed in E. Coli.
MA5-17195 detects TWIST1 which has a predicted molecular weight of approximately 21kDa.
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogensis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. TWIST also regulates cranial suture patterning and fusion and gene expression differentially depending on dimer composition. Mutations in the gene are implicated in Saethre-Chotzen syndrome, a disease characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-HLH DNA binding protein; bHLHa38; Class A basic helix-loop-helix protein 38; H-twist; p ACS3; twist basic helix-loop-helix transcription factor 1; twist homolog 1; TWIST homolog of drosophila; Twist-related protein 1
Gene Aliases: ACS3; BHLHA38; BPES2; BPES3; CRS; CRS1; CSO; SCS; TWIST; TWIST1
UniProt ID: (Human) Q15672
Entrez Gene ID: (Human) 7291
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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