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Invitrogen

TXK Polyclonal Antibody

View all (15) TXK antibodies

Datasheet

Protocols

Questions & Answers

Catalog # PA5-99845

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100 µL

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

TXK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

TXK Antibody (PA5-99845) in ICC/IF

Immunofluorescent analysis of TXK in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TXK polyclonal antibody (Product # PA5-99845) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

TXK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-99845

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human TXK(Accession P42681), corresponding to amino acid residues I30-V80.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2818778

Product Specific Information

Antibody detects endogenous levels of total TXK.

Target Information

TXK, along with the close relatives BMS, ITK, and BTK, signal downstream of antigen receptors and other types of receptors. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Protein-tyrosine kinase 4; PTK-RL-18; PTK4 protein tyrosine kinase 4; Resting lymphocyte kinase; tyrosine kinase; Tyrosine-protein kinase TXK

Gene Aliases: A130089B16Rik; BTKL; PSCTK5; PTK-RL-18; PTK4; RLK; TKL; TXK

UniProt ID: (Human) P42681, (Mouse) P42682

Entrez Gene ID: (Human) 7294, (Mouse) 22165

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding DNA binding protein kinase activity protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

cytokine production adaptive immune response regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway activation of phospholipase C activity integrin-mediated signaling pathway regulation of platelet activation cell differentiation positive regulation of interferon-gamma production peptidyl-tyrosine autophosphorylation regulation of cell proliferation tissue regeneration positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation T cell receptor signaling pathway positive regulation of interferon-gamma-mediated signaling pathway NK T cell differentiation immune system process transcription, DNA-templated regulation of transcription, DNA-templated phosphorylation interferon-gamma production interleukin-4 production innate immune response

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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