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Application
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Talin 1 Antibody (PA5-27646) in IHC (P)
Talin 1 antibody [C3], C-term detects TLN1 protein at cytosol on H357 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H357 xenograft. Talin 1 antibody [C3], C-term (Product # PA5-27646) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-27646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 2281 and 2537 of Human TLN1
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545122
Product Specific Information
Recommended positive controls: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (92%), Chicken (93%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Talin-1
Gene Aliases: ILWEQ; KIAA1027; TLN; TLN1
UniProt ID: (Human) Q9NZQ2, (Mouse) P26039
Entrez Gene ID: (Human) 7094, (Mouse) 21894
cytoskeletal anchoring at plasma membrane
cell-substrate junction assembly
cell adhesion
cortical actin cytoskeleton organization
platelet aggregation
platelet degranulation
movement of cell or subcellular component
muscle contraction
cell-cell junction assembly
viral process
IRE1-mediated unfolded protein response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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