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Bioss
Talin1 + 2 Polyclonal Antibody
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Application
FIGURE: 1 / 1
Talin1 + 2 Antibody (BS-3619R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with Talin Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB stainin... View More
Product Details
BS-3619R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Talin 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: talin 2; Talin-1; Talin-2
Gene Aliases: 5730421P04Rik; AI507121; AI787438; AL118320; ILWEQ; KIAA0320; KIAA1027; mKIAA0320; RGD1565416; TLN; TLN1; TLN2
UniProt ID: (Human) Q9NZQ2, (Human) Q9Y4G6, (Mouse) P26039, (Mouse) Q71LX4
Entrez Gene ID: (Human) 7094, (Human) 83660, (Rat) 313494, (Mouse) 21894, (Mouse) 70549, (Rat) 315776
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