Invitrogen

Thrombospondin 1 Monoclonal Antibody (C6.7)

Advanced Verification

IP-mass spectrometry

1 Published Figure

20 References

View all (21) Thrombospondin 1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Thrombospondin 1 Antibody in Western Blot (WB)

FIGURE: 1 / 4

Thrombospondin 1 Antibody (MA5-13390) in WB

Western blot of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13390) on HUVEC Cells.

Thrombospondin 1 Antibody in Immunoprecipitation (IP)

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Product Details

MA5-13390

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mL

Neutralization (Neu)

View 10 publications 10 publications

Functional Assay (Functional)

View 1 publication 1 publication

Immunomicroscopy (IM)

Assay-dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Human, Sheep, Pig, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C6.7

Immunogen

Ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979326

Product Specific Information

MA5-13390 targets Thrombospondin in FACS, IF, IM, IP, and WB applications and shows reactivity with Bovine, Canine, Equine, Human, Ovine, Porcine, and Rat samples.

The MA5-13390 immunogen is ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets.

Target Information

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme.

eLife

Jacquemin G,Wurmser A,Huyghe M,Sun W,Homayed Z,Merle C,Perkins M,Qasrawi F,Richon S,Dingli F,Arras G,Loew D,Vignjevic D,Pannequin J,Fre S

MA5-13390 was used in Neutralization experiments to reveal the THBS1-YAP axis as the mechanistic link mediating paracrine interactions between epithelial cells in intestinal tumours.

Wed May 11 00:00:00 EDT 2022

The 10q26 Risk Haplotype of Age-Related Macular Degeneration Aggravates Subretinal Inflammation by Impairing Monocyte Elimination.

Immunity

Beguier F,Housset M,Roubeix C,Augustin S,Zagar Y,Nous C,Mathis T,Eandi C,Benchaboune M,Drame-Maigné A,Carpentier W,Chardonnet S,Touhami S,Blot G,Conart JB,Charles-Messance H,Potey A,Girmens JF,Paques M,Blond F,Leveillard T,Koertvely E,Roger JE,Sahel JA,Sapieha P,Delarasse C,Guillonneau X,Sennlaub F

MA5-13390 was used in Inhibition Assays to study the mechanisms whereby the risk 10q26 haplotype promotes age-related macular degeneration.

Tue Aug 18 00:00:00 EDT 2020

Adenosine stimulates angiogenesis by up-regulating production of thrombospondin-1 by macrophages.

Journal of leukocyte biology

Ernens I,Bousquenaud M,Lenoir B,Devaux Y,Wagner DR

MA5-13390 was used in Functional assay to determine whether Ado modulates TSP-1 expression and the implication on angiogenesis.

Thu Jan 01 00:00:00 EST 2015

Adenosine stimulates angiogenesis by up-regulating production of thrombospondin-1 by macrophages.

Journal of leukocyte biology

Ernens I,Bousquenaud M,Lenoir B,Devaux Y,Wagner DR

MA5-13390 was used in Functional assay to determine whether Ado modulates TSP-1 expression and the implication on angiogenesis.

Thu Jan 01 00:00:00 EST 2015

Adipose-derived stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the formation of microvascular networks.

Experimental gerontology

Aird AL,Nevitt CD,Christian K,Williams SK,Hoying JB,LeBlanc AJ

MA5-13390 was used in western blot to study the effect of advancing age on the capacity of the stromal vascular fraction to stimulate neovascularization.

Sun Mar 01 00:00:00 EST 2015

Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.

Immunology

Talme T,Bergdahl E,Sundqvist KG

MA5-13390 was used in western blot to study the roles of LRP-1 and thrombospondin -1 in regulating the motility and adhesion/de-adhesion of T-cells

Sun Jun 01 00:00:00 EDT 2014

A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.

Immunology

Bergström SE,Bergdahl E,Sundqvist KG

MA5-13390 was used in blocking or activating experiment and western blot to study the mechanism underlying cytokine-mediated modulation of the motility, adhesion and activation of T-cells

Sun Dec 01 00:00:00 EST 2013

Bystander senescence in human peritoneal mesothelium and fibroblasts is related to thrombospondin-1-dependent activation of transforming growth factor-β1.

The international journal of biochemistry & cell biology

Mikuła-Pietrasik J,Sosińska P,Janus J,Rubiś B,Brewińska-Olchowik M,Piwocka K,Książek K

MA5-13390 was used in ELISA to study the role of thrombospondin-1 and TGF-beta1 in bystander senescence in human peritoneal mesothelium and fibroblasts

Sun Sep 01 00:00:00 EDT 2013

Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.

The Journal of biological chemistry

Ikeda H,Miyatake M,Koshikawa N,Ochiai K,Yamada K,Kiss A,Donlin MJ,Panneton WM,Churchill JD,Green M,Siddiqui AM,Leinweber AL,Crews NR,Ezerskiy LA,Rendell VR,Belcheva MM,Coscia CJ

Published figure using Thrombospondin 1 monoclonal antibody (Product # MA5-13390) in Western Blot

Fri Dec 03 00:00:00 EST 2010

Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.

The Journal of biological chemistry

Ikeda H,Miyatake M,Koshikawa N,Ochiai K,Yamada K,Kiss A,Donlin MJ,Panneton WM,Churchill JD,Green M,Siddiqui AM,Leinweber AL,Crews NR,Ezerskiy LA,Rendell VR,Belcheva MM,Coscia CJ

Published figure using Thrombospondin 1 monoclonal antibody (Product # MA5-13390) in Western Blot

Fri Dec 03 00:00:00 EST 2010

Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain.

Translational research : the journal of laboratory and clinical medicine

Gayen Betal S,Setty BN

MA5-13390 was used in blocking or activating experiment to investigate the role of the heparin-binding domain of the phosphatidylserine-positive erythrocytes in its binding to immobilized and soluble thrombosphodin-1

Wed Oct 01 00:00:00 EDT 2008

Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations.

The Journal of biological chemistry

Annis DS,Gunderson KA,Mosher DF

MA5-13390 was used in ELISA to probe the structure of thrombospondin-1 and thrombospondin-2 signature domains at low calcium concentration using conformation-sensitive monoclonal antibodies

Fri Sep 14 00:00:00 EDT 2007

Thrombospondin-1 and indoleamine 2,3-dioxygenase are major targets of extracellular ATP in human dendritic cells.

Blood

Marteau F,Gonzalez NS,Communi D,Goldman M,Boeynaems JM,Communi D

MA5-13390 was used in blocking or activating experiment to investigate the effect of ATP on the expression of the indoleamine 2,3-dioxygenase and kynurenine production in human dendritic cells

Thu Dec 01 00:00:00 EST 2005

Novel CD47-dependent intercellular adhesion modulates cell migration.

Journal of cellular physiology

Rebres RA,Kajihara K,Brown EJ

MA5-13390 was used in blocking or activating experiment to study cell migration modulated by CD47-dependent intercellular adhesion

Tue Nov 01 00:00:00 EST 2005

Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.

Journal of immunology (Baltimore, Md. : 1950)

Li SS,Forslöw A,Sundqvist KG

MA5-13390 was used in immunocytochemistry to study the role of the calreticulin-thrombospondin-1 interaction in autocrine regulation of T cell motility

Sat Jan 15 00:00:00 EST 2005

Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.

The Journal of biological chemistry

Emonard H,Bellon G,Troeberg L,Berton A,Robinet A,Henriet P,Marbaix E,Kirkegaard K,Patthy L,Eeckhout Y,Nagase H,Hornebeck W,Courtoy PJ

MA5-13390 was used in blocking or activating experiment to investigate the mechanism of endocytic clearance mediated by low density lipoprotein receptor-related protein

Fri Dec 24 00:00:00 EST 2004

Thrombospondin 1 is an autocrine negative regulator of human dendritic cell activation.

The Journal of experimental medicine

Doyen V,Rubio M,Braun D,Nakajima T,Abe J,Saito H,Delespesse G,Sarfati M

MA5-13390 was used in flow cytometry to investigate the role of thrombospondin 1 in human dendritic cell activation

Mon Oct 20 00:00:00 EDT 2003

Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.

Molecular and cellular biology

Lopes N,Gregg D,Vasudevan S,Hassanain H,Goldschmidt-Clermont P,Kovacic H

MA5-13390 was used in immunocytochemistry to investigate the relationship between thrombospondin 2 and Rac1

Fri Aug 01 00:00:00 EDT 2003

CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum.

Infection and immunity

Smith TG,Serghides L,Patel SN,Febbraio M,Silverstein RL,Kain KC

MA5-13390 was used in blocking or activating experiment to study the role of CD36 in the non-opsonic phagocytosis of erythrocytes infected with Plasmodium falciparum gametocytes

Wed Jan 01 00:00:00 EST 2003

Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.

Blood

Goel MS,Diamond SL

MA5-13390 was used in blocking or activating experiment to investigate the adhesion of erythrocytes during deep vein thrombosis

Fri Nov 15 00:00:00 EST 2002

Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.

Biochimica et biophysica acta

Gupta K,Gupta P,Solovey A,Hebbel RP

MA5-13390 was used in blocking or activating experiment to study the mechanism by which thrombospondin mediates the adhesion of sickle erythrocytes to endothelium

Wed Jan 06 00:00:00 EST 1999

Bioinformatics

Protein Aliases: Glycoprotein G; Thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1

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Gene Aliases: THBS; THBS-1; THBS1; TSP; TSP-1; TSP1

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UniProt ID: (Human) P07996, (Bovine) Q28178

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Entrez Gene ID: (Horse) 100057478, (Dog) 487486, (Human) 7057, (Bovine) 281530, (Pig) 492313, (Sheep) 101106433, (Rat) 445442

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Function(s)

phosphatidylserine binding glycoprotein binding fibronectin binding integrin binding calcium ion binding protein binding heparin binding fibroblast growth factor binding low-density lipoprotein particle binding identical protein binding laminin binding proteoglycan binding transforming growth factor beta binding fibrinogen binding collagen V binding extracellular matrix binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Thrombospondin 1 Monoclonal Antibody (C6.7)

Thrombospondin 1 Antibody (MA5-13390) in WB

Product Details

MA5-13390

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Immunomicroscopy (IM)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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