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Toca-1 Antibody (A303-469A) in IP
Detection of human Toca-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Toca-1 antibody (Product # A303-469A) used for IP at 6 µg/mg lysate. Toca-1 was also immunoprecipitated by rabbit anti-Toca-1 antibody A303-470A, which recognizes a downstream epitope. For blotting immunoprecipitated Toca-1, A303-470A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-469A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 and 100 of Human Transducer of Cdc42-Dependent Actin Assembly Protein 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.^M
^M
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: formin binding protein 1-like; Formin-binding protein 1-like; Toca-1; transducer of Cdc42-dependent actin assembly 1; Transducer of Cdc42-dependent actin assembly protein 1
Gene Aliases: C1orf39; FNBP1L; TOCA1
UniProt ID: (Human) Q5T0N5
Entrez Gene ID: (Human) 54874
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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