Loading ...

Invitrogen

Ty1 Tag Monoclonal Antibody (BB2)

11 References

View all (2) Ty1 Tag antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Catalog # MA5-23513

Price (EUR)

493,00

50 µg

Check your price

Request bulk or custom format

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)

Application

FIGURE: 1 / 3

Ty1 Tag Antibody (MA5-23513) in WB

Western Blot was performed on 20 µg whole cell extracts from MCF7 cells using a Ty1 Tag monoclonal antibody (Product # MA5-23513) at a dilution of 1:2,000 in TBS-Tween containing 3% skimmed milk. The figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a Ty1-tagged protein. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left. The smaller bands present on the blot represent degradation product of the overexpressed protein.

Product Image Gallery

Product Details

MA5-23513

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:15,000

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

ChIP assay (ChIP)

2 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BB2

Immunogen

Ty1 tag (amino acid sequence EVHTNQDPLD).

Conjugate

Unconjugated

Form

Liquid

Concentration

1.9 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2610644

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The Ty1 Tag recognizes Ty1-tagged proteins: EVHTNQDPLD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recombinant cellular model system for human muscle-type nicotinic acetylcholine receptor α1₂β1δε.

Cell stress & chaperones

Brockmöller S,Seeger T,Worek F,Rothmiller S

MA5-23513 was used in Western Blot to present a stable and functional cell line that expresses human muscle-type nAChR and yields can be further increased using the chemical chaperone nicotine without affecting cell viability.

Wed Nov 01 00:00:00 UTC 2023

Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila.

Nature communications

Shekhar S,Moehlman AT,Park B,Ewnetu M,Tracy C,Titos I,Pawłowski K,Tagliabracci VS,Krämer H

MA5-23513 was used in Western Blot to identify the conserved Allnighter (Aln) pseudokinase as a cell non-autonomous regulator of proteostasis responses necessary for normal sleep and structural plasticity of Drosophila photoreceptors.

Mon May 22 00:00:00 UTC 2023

Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila.

Nature communications

Shekhar S,Moehlman AT,Park B,Ewnetu M,Tracy C,Titos I,Pawłowski K,Tagliabracci VS,Krämer H

Mon May 22 00:00:00 UTC 2023

A phosphoswitch at acinus-serine⁴³⁷ controls autophagic responses to cadmium exposure and neurodegenerative stress.

eLife

Nandi N,Zaidi Z,Tracy C,Krämer H

MA5-23513 was used in Western Blot, Immunocytochemistry to establish the acn-S437 phosphoswitch as critical integrator of multiple stress signals regulating neuronal autophagy.

Mon Jan 17 00:00:00 UTC 2022

A phosphoswitch at acinus-serine⁴³⁷ controls autophagic responses to cadmium exposure and neurodegenerative stress.

eLife

Nandi N,Zaidi Z,Tracy C,Krämer H

MA5-23513 was used in Western Blot, Immunocytochemistry to establish the acn-S437 phosphoswitch as critical integrator of multiple stress signals regulating neuronal autophagy.

Mon Jan 17 00:00:00 UTC 2022

Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML.

Molecular cell

Riedel SS,Lu C,Xie HM,Nestler K,Vermunt MW,Lenard A,Bennett L,Speck NA,Hanamura I,Lessard JA,Blobel GA,Garcia BA,Bernt KM

MA5-23513 was used in Western Blot, RNA Immunoprecipitation, Immunocytochemistry to detail how the overexpression of a polyQ protein, in the absence of any coding sequence mutation, can be sufficient to cause malignant transformation.

Thu Jun 03 00:00:00 UTC 2021

Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML.

Molecular cell

Riedel SS,Lu C,Xie HM,Nestler K,Vermunt MW,Lenard A,Bennett L,Speck NA,Hanamura I,Lessard JA,Blobel GA,Garcia BA,Bernt KM

Thu Jun 03 00:00:00 UTC 2021

Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML.

Molecular cell

Riedel SS,Lu C,Xie HM,Nestler K,Vermunt MW,Lenard A,Bennett L,Speck NA,Hanamura I,Lessard JA,Blobel GA,Garcia BA,Bernt KM

Thu Jun 03 00:00:00 UTC 2021

Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions.

PLoS pathogens

Wong ZS,Sokol-Borrelli SL,Olias P,Dubey JP,Boyle JP

MA5-23513 was used in Immunocytochemistry to suggest that T. gondii manipulation of the host cell is capable of suppressing previously unknown stress and/or DNA-damage induced responses that occur during infection with H. hammondi, and that one important impact of this T. gondii mediated suppression is to promote parasite replication.

Mon Jun 01 00:00:00 UTC 2020

Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration.

eLife

Broncel M,Dominicus C,Vigetti L,Nofal SD,Bartlett EJ,Touquet B,Hunt A,Wallbank BA,Federico S,Matthews S,Young JC,Tate EW,Tardieux I,Treeck M

MA5-23513 was used in Western Blot, Immunocytochemistry to describe the myristoylated proteome of Toxoplasma gondii using chemoproteomic methods and show that a small-molecule NMT inhibitor developed against related Plasmodium spp. is also functional in Toxoplasma.

Fri Jul 03 00:00:00 UTC 2020

Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration.

eLife

Broncel M,Dominicus C,Vigetti L,Nofal SD,Bartlett EJ,Touquet B,Hunt A,Wallbank BA,Federico S,Matthews S,Young JC,Tate EW,Tardieux I,Treeck M

Fri Jul 03 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Ty1 Epitope Tag

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Ty1 Tag Monoclonal Antibody (BB2)

Ty1 Tag Antibody (MA5-23513) in WB

Product Details

MA5-23513

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!