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GenScript

Ty1-tag Polyclonal Antibody

3 References

View all (2) Ty1 Tag antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

Application

FIGURE: 1 / 2

Ty1-tag Antibody (A01004-40) in WB

Western blot analysis of Ty1 tagged fusion proteins expressed in E. coli cell lysate using Ty1-tag Antibody (GenScript, A01004, 1 µg/mL). The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

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Product Details

A01004-40

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Immunoprecipitation (IP)

2-5 µg/mL

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Ty1-tag epitope peptide conjugated to KLH

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Well-characterized antibodies for epitope tags are common in the study of protein expression in various systems.GenScript Rabbit Anti- Ty1-tag Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The Ty1 Tag recognizes Ty1-tagged proteins: EVHTNQDPLD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TET1 is a beige adipocyte-selective epigenetic suppressor of thermogenesis.

Nature communications

Damal Villivalam S,You D,Kim J,Lim HW,Xiao H,Zushin PH,Oguri Y,Amin P,Kang S

Thu Aug 27 00:00:00 UTC 2020

Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania.

Nature communications

Bhattacharya A,Leprohon P,Bigot S,Padmanabhan PK,Mukherjee A,Roy G,Gingras H,Mestdagh A,Papadopoulou B,Ouellette M

Current genome-wide screens allow system-wide study of drug resistance but detecting small nucleotide variants (SNVs) is challenging. Here, we use chemical mutagenesis, drug selection and next generation sequencing to characterize miltefosine and paromomycin resistant clones of the parasite Leishmania. We highlight several genes involved in drug resistance by sequencing the genomes of 41 resistant clones and by concentrating on recurrent SNVs. We associate genes linked to lipid metabolism or to

Mon Dec 09 00:00:00 UTC 2019

Essential cGMP Signaling in Toxoplasma Is Initiated by a Hybrid P-Type ATPase-Guanylate Cyclase.

Cell host & microbe

Brown KM,Sibley LD

Apicomplexan parasites rely on cyclic nucleotide-dependent kinases for host cell infection yet the mechanisms that control their activation remain unknown. Here we show that an apically localized guanylate cyclase (GC) controls microneme secretion and lytic growth in the model apicomplexan Toxoplasma gondii. Cell-permeable cGMP reversed the block in microneme secretion seen in a knockdown of TgGC linking its function to production of cGMP. TgGC possesses an N-terminal P-type ATPase domain fused

Wed Dec 12 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Ty1 Epitope Tag

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