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Application
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UBC9 Antibody (PA5-17043) in WB
Western blot was performed using Anti-UBC9 Polyclonal Antibody (Product # PA5-17043) and a 18 kDa band corresponding to UBC9 was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep-G2 (Lane 2), MCF7 (Lane 3), BJ (Lane 4), MDA-MB-231 (Lane 5), 3T3-L1 (Lane 6), 3T3-L1 differentiated to adipocytes (Lane 7), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8), Rat Spleen (Lane 9), Mouse Thymus (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE® 4-12 % Bis-Tris g... View More
Product Details
PA5-17043
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding V148 of human Ubc9
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985536
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mUBC9; OTTHUMP00000198131; p18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); Ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I
Gene Aliases: 5830467E05Rik; C358B7.1; F830028O17Rik; P18; UBC9; UbcE2A; Ubce2i; UBCE9; UBE2I
UniProt ID: (Human) P63279, (Rat) P63281, (Mouse) P63280
Entrez Gene ID: (Human) 7329, (Rat) 25573, (Mouse) 22196
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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