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Invitrogen
UBE1L Recombinant Superclonal Antibody (7HCLC)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
UBE1L Antibody (712170) in WB
Immunoblot with UBE1L antibody (Product # 712170). NK-92, HeLa, Jurkat, and A549 cells were either untreated or IFN-b-treated for 48 hours as indicated. 8220Transfected8221 (last lane) represents extract from HEK293T cells transfected with plasmids expressing 3X-FLAG-ISG15, UBE1L, UBCH8, and HERC5. Cells were lysed in 1% NP40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 1% NP-40) with PMSF, aprotinin, leupeptin, and 1mM DTT. 30 mg total protein per sample were loaded onto a 13% Tris-Glycine PAGE gel, transferred to nitrocellulose using a se... View More
Product Details
712170
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
Protein corresponding to human UBE1L [aa880-aa1012]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784613
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Horse, Cat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
UBE1L (ubiquitin-like modifier-activating enzyme 7) activates ubiquitin by first adenylating with ATP at its C-terminal glycine residue and then linking that residue to the side chain of a cysteine residue in E1. This activation results in an ubiquitin-E1 thioester and free AMP. UBE1L also catalyzes the ISGylation of influenza A virus NS1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D8; MGC12713; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7
Gene Aliases: D8; UBA1B; UBA7; UBE1L; UBE2
UniProt ID: (Human) P41226
Entrez Gene ID: (Human) 7318
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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