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UBE2C Antibody (MA5-47921) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of UBE2C in Caco-2 cells using UBE2C Recombinant Monoclonal Antibody (Product # MA5-47921). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton and stained with UBE2C Recombinant Monoclonal Antibody at a dilution of 1:100 for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom UBE2C Recombinant Monoclonal Antibody, DAPI, merged channels and an isotype contr... View More
Product Details
MA5-47921
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SAIC-41A-1
Immunogen
Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074365
Product Specific Information
Specificity: Recognises human UBE2C.
Target Information
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme C; cyclin-selective ubiquitin carrier protein; E2 ubiquitin-conjugating enzyme C; mitotic-specific ubiquitin-conjugating enzyme; RP3-447F3.1; UbcH10; Ubiquitin carrier protein C; ubiquitin conjugating enzyme E2C; Ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C
Gene Aliases: dJ447F3.2; UBCH10; UBE2C
UniProt ID: (Human) O00762
Entrez Gene ID: (Human) 11065
ubiquitin-dependent protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation
exit from mitosis
free ubiquitin chain polymerization
protein ubiquitination
regulation of mitotic metaphase/anaphase transition
anaphase-promoting complex-dependent catabolic process
positive regulation of exit from mitosis
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
protein K48-linked ubiquitination
protein K11-linked ubiquitination
positive regulation of ubiquitin protein ligase activity
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