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FIGURE: 1 / 7
UBE4A Antibody (MA5-44916) in ICC/IF
Immunocytochemistry analysis of Ube4A in SK-OV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in Ube4A Monoclonal antibody (Product # MA5-44916) using a dilution of 1:50 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000. The nuclear counter stain is DAPI (blue).
Product Details
MA5-44916
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-68
Immunogen
Recombinant protein within human Ube4A aa 1-100.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931372
Target Information
Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins. Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4. In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RING-type E3 ubiquitin transferase E4 A; Ubiquitin conjugation factor E4 A; ubiquitination factor E4A (UFD2 homolog, yeast); ubiquitination factor E4A, UFD2 homolog
Gene Aliases: Ab2-232; E4; KIAA0126; UBE4A; UBOX2; UFD2
UniProt ID: (Human) Q14139, (Rat) Q6P7A2
Entrez Gene ID: (Human) 9354, (Rat) 315608
Performance Guarantee
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