Proteintech

ULK1 Polyclonal Antibody

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ULK1 Antibody (20986-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

20986-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 45 publications 45 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1

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Gene Aliases: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1

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UniProt ID: (Human) O75385, (Mouse) O70405

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Entrez Gene ID: (Human) 8408, (Rat) 360827, (Mouse) 22241

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Function(s)

protein serine/threonine kinase activity protein binding ATP binding Rab GTPase binding protein complex binding molecular_function protein kinase activity protein kinase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Hsp90 protein binding non-receptor serine/threonine protein kinase

Process(es)

protein phosphorylation signal transduction protein localization regulation of autophagy positive regulation of autophagy macroautophagy positive regulation of macroautophagy peptidyl-serine phosphorylation neuron projection regeneration neuron projection development negative regulation of protein complex assembly cellular response to nutrient levels response to starvation protein autophosphorylation axon extension autophagosome assembly Ras protein signal transduction axonogenesis cerebellar granule cell differentiation radial glia guided migration of cerebellar granule cell receptor internalization negative regulation of collateral sprouting regulation of neurotrophin TRK receptor signaling pathway autophagy regulation of gene expression regulation of cell death phosphorylation mitophagy in response to mitochondrial depolarization

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ULK1 Polyclonal Antibody

ULK1 Antibody (20986-1-AP) in IHC (P)

Product Details

20986-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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