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Application
FIGURE: 1 / 2
ULK2 Antibody (PA5-77808) in ICC/IF
Immunofluorescent analysis of ULK2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ULK2 polyclonal antibody (Product # PA5-77808) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ULK2 Antibody D) Merged image. Magnification: 60x.
Product Details
PA5-77808
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the mid-protein of Human ULK2 (aa 558-569)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2736767
Product Specific Information
A 1:1,000 dilution of PA5-77808 was sufficient for detection of ULK2 in 15 µg of Human HeLa Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 100 kDa.
Target Information
ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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