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Bethyl Laboratories
UNG Polyclonal Antibody
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Application
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UNG Antibody (A302-888A) in WB
Detection of human UNG by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody (Product # A302-888A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody A302-887A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B)... View More
Product Details
A302-888A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 263 and 313 of Human Uracil-DNA Glycosylase.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB. The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis. Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type. Additionally, the expression of UNG is cell cycle regulated. The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells. UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781L1143; UDG; UNG; Uracil-DNA glycosylase; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
Gene Aliases: DGU; HIGM4; HIGM5; UDG; UNG; UNG1; UNG15; UNG2
UniProt ID: (Human) P13051
Entrez Gene ID: (Human) 7374
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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