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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • USP3 Antibodies

            Bethyl Laboratories

            USP3 Polyclonal Antibody

            View all (12) USP3 antibodies
            Datasheet
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            Datasheet
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            Cite USP3 Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            USP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            USP3 Antibody (A301-541A) in IHC (P)

            Detection of human USP3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-USP3 (Product # A301-541A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
            View Product

            USP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            USP3 Antibody in Immunoprecipitation (IP)

            Product Details

            A301-541A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            Assay-dependent
            -

            Immunohistochemistry (IHC)

            1:1,000-1:5,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:1,000-1:5,000
            -

            Immunoprecipitation (IP)

            2-5 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 470 and 520 of Human ubiquitin specific protease 3.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            tris citrate/phosphate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

            Target Information

            Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: Deubiquitinating enzyme 3; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin thioesterase 3; ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3

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            Gene Aliases: SIH003; UBP; USP3

            View more View less

            UniProt ID: (Human) Q9Y6I4

            View more View less

            Entrez Gene ID: (Human) 9960

            View more View less

            Function(s)

            thiol-dependent ubiquitin-specific protease activity zinc ion binding histone binding cysteine protease

            Process(es)

            mitotic cell cycle DNA repair ubiquitin-dependent protein catabolic process histone deubiquitination regulation of protein stability
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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