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Application
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USP9X Antibody (PA5-143421) in IHC (PFA)
Immunohistochemistry analysis of USP9X in 1 day-post-fertilization zebrafish embryo (whole mount). Samples were incubated in USP9X Polyclonal antibody (Product # PA5-143421) using a dilution of 1:100.
Product Details
PA5-143421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus of zebrafish Usp9. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.81 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2938416
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Positive control: brain, liver, ovary, muscle.
Target Information
USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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