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Invitrogen

VAMP2 Polyclonal Antibody

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2 Published Figures

9 References

View all (18) VAMP2 antibodies

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Protocols

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VAMP2 Antibody (PA1-766) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Lung (Lane 3), Rat Lung (Lane 4), Rat Liver (Land 5) and Rat Kidney (Lane 6). The blot was probed with Anti- VAMP2 Rabbit Polyclonal Antibody (Product # PA1-766, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to VAMP2 was observed in Mouse Brain, Rat Brain and no... View More

VAMP2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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VAMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP2 was observed in Mouse Brain, Rat Brain, Mouse Lung, Rat Lung, Rat Liver and Rat Kidney in Western Blot using VAMP2 Polyclonal Antibody (Product # PA1-766). VAMP2 is reported to be expressed in brain tissue and not in other tissues like lung, liver and kidney. {RE}

Product Details

PA1-766

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2304164

Product Specific Information

PA1-766 detects vesicle associated membrane protein 2 (VAMP-2) from rat and mouse tissues.

PA1-766 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19 kDa protein representing VAMP-2 from rat brain whole protein extract.

The PA1-766 immunogen is a synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-101) is available for use in neutralization and control experiments.

Target Information

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.

The Journal of biological chemistry

Brasher MI,Martynowicz DM,Grafinger OR,Hucik A,Shanks-Skinner E,Uniacke J,Coppolino MG

Published figure using VAMP2 polyclonal antibody (Product # PA1-766) in Immunoprecipitation

Fri Sep 29 00:00:00 UTC 2017

Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.

The Journal of biological chemistry

Brasher MI,Martynowicz DM,Grafinger OR,Hucik A,Shanks-Skinner E,Uniacke J,Coppolino MG

Published figure using VAMP2 polyclonal antibody (Product # PA1-766) in Immunoprecipitation

Fri Sep 29 00:00:00 UTC 2017

Cellular compartmentation of energy metabolism: creatine kinase microcompartments and recruitment of B-type creatine kinase to specific subcellular sites.

Amino acids

Schlattner U,Klaus A,Ramirez Rios S,Guzun R,Kay L,Tokarska-Schlattner M

PA1-766 was used in western blot to study creatine kinase microcompartments and energy metabolism that recruits B-type creatine kinase to specific subcellular sites

Mon Aug 01 00:00:00 UTC 2016

Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics.

Journal of proteome research

El Ouaamari A,Zhou JY,Liew CW,Shirakawa J,Dirice E,Gedeon N,Kahraman S,De Jesus DF,Bhatt S,Kim JS,Clauss TR,Camp DG,Smith RD,Qian WJ,Kulkarni RN

PA1-766 was used in western blot to identify alterations in the islet proteome that characterize the adaptive response

Fri Aug 07 00:00:00 UTC 2015

The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.

American journal of physiology. Endocrinology and metabolism

Szekeres F,Chadt A,Tom RZ,Deshmukh AS,Chibalin AV,Björnholm M,Al-Hasani H,Zierath JR

PA1-766 was used in western blot to study the role of the Rab-GTPase-activating protein TBC1D1 in regulating skeletal muscle glucose metabolism

Wed Aug 15 00:00:00 UTC 2012

American journal of physiology. Endocrinology and metabolism

Niu W,Bilan PJ,Ishikura S,Schertzer JD,Contreras-Ferrat A,Fu Z,Liu J,Boguslavsky S,Foley KP,Liu Z,Li J,Chu G,Panakkezhum T,Lopaschuk GD,Lavandero S,Yao Z,Klip A

PA1-766 was used in western blot to develop a cellular model for glucose uptake following muscle contraction

Sat May 01 00:00:00 UTC 2010

Cleavage of SNAP-25 and VAMP-2 impairs store-operated Ca2+ entry in mouse pancreatic acinar cells.

American journal of physiology. Cell physiology

Rosado JA,Redondo PC,Salido GM,Sage SO,Pariente JA

PA1-766 was used in western blot to demonstrate the role of SNAP-25 and VAMP-2 in the activation of SOCE in pancreatic acinar cells

Sat Jan 01 00:00:00 UTC 2005

Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells.

Molecular endocrinology (Baltimore, Md.)

Marshall C,Hitman GA,Partridge CJ,Clark A,Ma H,Shearer TR,Turner MD

PA1-766 was used in western blot to study the function ofa calpain-10 isoform as an exocytosis trigger in pancreatic beta-cells.

Sat Jan 01 00:00:00 UTC 2005

The septin CDCrel-1 is dispensable for normal development and neurotransmitter release.

Molecular and cellular biology

Peng XR,Jia Z,Zhang Y,Ware J,Trimble WS

PA1-766 was used in immunofluorescence to probe VAMP-2 in primary cultures of mouse hippocampal neurons.

Tue Jan 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: Synaptobrevin; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2

Gene Aliases: RATVAMPB; RATVAMPIR; SYB; Syb-2; Syb2; sybII; Vamp2

UniProt ID: (Mouse) P63044, (Rat) P63045

Entrez Gene ID: (Mouse) 22318, (Rat) 24803

Function(s)

SNARE binding SNAP receptor activity protein binding calmodulin binding phospholipid binding protein C-terminus binding myosin binding syntaxin-1 binding syntaxin binding protein complex binding identical protein binding ion channel binding calcium-dependent protein binding

Process(es)

protein complex assembly vesicle fusion response to glucose protein transport synaptic vesicle exocytosis vesicle-mediated transport calcium ion regulated exocytosis regulation of exocytosis regulation of calcium ion-dependent exocytosis cellular response to insulin stimulus Golgi to plasma membrane protein transport eosinophil degranulation long-term synaptic potentiation regulation of vesicle-mediated transport membrane fusion positive regulation of intracellular protein transport regulation of delayed rectifier potassium channel activity neurotransmitter secretion neutrophil degranulation natural killer cell degranulation mucus secretion regulation of histamine secretion by mast cell

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VAMP2 Polyclonal Antibody

VAMP2 Antibody (PA1-766) in WB

Product Details

PA1-766

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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VAMP2 Polyclonal Antibody

VAMP2 Antibody (PA1-766) in WB

Product Details

PA1-766

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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