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            • Primary Antibodies ›
            • DDX4 Antibodies

            AbboMax

            VASA Polyclonal Antibody

            View all (42) DDX4 antibodies
            Datasheet
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            Datasheet
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            Cite VASA Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            VASA Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            VASA Antibody (601-360) in WB

            Western Blot: The cell lysate derived from mouse embryonic germ cell (20 µg/lane) was immunoprobed by Rabbit anti-VASA antibody (Cat#601-360) at 1:500. An immunoreactive band is observed around ~76 kDa (lane 1). This band is depleted by pre-incubation with immunizing peptide (Lane 2).
            View Product

            VASA Antibody in Western Blot (WB)

            Product Details

            601-360

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.1-1 µg/mL
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            2-10 µg/mL
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthetic peptide corresponding to the N-terminus of human VASA protein
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with proprietary stabilizer

            Contains

            0.01% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Positive control: Mouse embryonic germ line cell

            Cellular location: Cytoplasmic.

            Target Information

            DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; Probable ATP-dependent RNA helicase DDX4; Vasa homolog

            View more View less

            Gene Aliases: DDX4; VASA

            View more View less

            UniProt ID: (Human) Q9NQI0

            View more View less

            Entrez Gene ID: (Human) 54514

            View more View less

            Function(s)

            nucleic acid binding ATP-dependent RNA helicase activity ATP binding

            Process(es)

            male meiosis multicellular organism development spermatogenesis RNA secondary structure unwinding cell differentiation sperm motility gene silencing by RNA piRNA metabolic process DNA methylation involved in gamete generation
            It has to be done as per old AB suggested Products section.
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