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OriGene

VHL Monoclonal Antibody (OTI1E1), TrueMAB™

View all (22) VHL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

Application

VHL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

VHL Antibody (TA506222) in IHC (P)

Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506222)

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Product Details

TA506222

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI1E1

Immunogen

Full length human recombinant protein of human VHL produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5-1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: elongin binding protein; Protein G7; pVHL; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase

Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1

UniProt ID: (Human) P40337

Entrez Gene ID: (Human) 7428

Function(s)

ubiquitin-protein transferase activity protein binding transcription factor binding enzyme binding ubiquitin protein ligase activity ubiquitin-protein ligase

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis regulation of transcription, DNA-templated proteolysis negative regulation of cell proliferation protein ubiquitination negative regulation of apoptotic process positive regulation of cell differentiation positive regulation of transcription, DNA-templated protein stabilization regulation of transcription from RNA polymerase II promoter in response to hypoxia negative regulation of transcription from RNA polymerase II promoter in response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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