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OriGene

VIL1 Monoclonal Antibody (OTI3B3), TrueMAB™

1 Reference

View all (55) Villin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

FIGURE: 1 / 1

VIL1 Antibody (TA803236) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIL1 (RC216340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-VIL1. Positive lysates LY416173 (100 µg) and LC416173 (20 µg) can be purchased separately from OriGene.

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Product Details

TA803236

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3B3

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-188 of human VIL1 produced in E.coli.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HpSlyD inducing CDX2 and VIL1 expression mediated through TCTP protein may contribute to intestinal metaplasia in the stomach.

Scientific reports

Li Q,Zhu Y,Liu J,Yu X,Chen M,Dong N,Gong Y,Yuan Y

Tue May 23 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: OTTHUMP00000207159; Villin-1

Gene Aliases: D2S1471; VIL; VIL1

UniProt ID: (Human) Q53S11

Entrez Gene ID: (Human) 7429

Function(s)

calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding lysophosphatidic acid binding identical protein binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process actin filament binding non-motor actin binding protein

Process(es)

intestinal D-glucose absorption protein complex assembly apoptotic process epidermal growth factor receptor signaling pathway regulation of cell shape response to bacterium positive regulation of epithelial cell migration actin filament polymerization actin filament depolymerization positive regulation of cell migration positive regulation of actin filament depolymerization epithelial cell differentiation positive regulation of actin filament bundle assembly regulation of microvillus length cellular response to hepatocyte growth factor stimulus positive regulation of multicellular organism growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process actin nucleation actin filament severing barbed-end actin filament capping regulation of actin nucleation actin filament capping cytoplasmic actin-based contraction involved in cell motility regulation of wound healing cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane terminal web assembly regulation of lamellipodium morphogenesis positive regulation of lamellipodium morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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