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VPS11 Antibody (A303-528A) in WB
Detection of human VPS11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-VPS11 antibody (Product # A303-528A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. VPS11 was also immunoprecipitated by rabbit anti-VPS11 antibody A303-527A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A)... View More
Product Details
A303-528A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 891 and 941 of Human Vacuolar Protein Sorting 11.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hVPS11; RING finger protein 108; vacuolar protein sorting 11 homolog; Vacuolar protein sorting-associated protein 11 homolog; VPS11 CORVET/HOPS core subunit
Gene Aliases: END1; HLD12; hVPS11; PEP5; PP3476; RNF108; VPS11
UniProt ID: (Human) Q9H270
Entrez Gene ID: (Human) 55823
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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